Coacervation of resilin fusion proteins containing terminal functionalities
- VTT Technical Research Centre of Finland
Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.
|Julkaisu||Colloids and Surfaces B: Biointerfaces|
|Tila||Julkaistu - 1 marraskuuta 2018|
|OKM-julkaisutyyppi||A1 Julkaistu artikkeli, soviteltu|