Coacervation of resilin fusion proteins containing terminal functionalities

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

Organisaatiot

  • VTT Technical Research Centre of Finland

Kuvaus

Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut590-596
Sivumäärä7
JulkaisuColloids and Surfaces B: Biointerfaces
Vuosikerta171
TilaJulkaistu - 1 marraskuuta 2018
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 27193430