Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

  • Ndegwa Henry Maina
  • Leena Pitkänen

  • Sami Heikkinen
  • Päivi Tuomainen
  • Liisa Virkki
  • Maija Tenkanen

Organisaatiot

  • University of Helsinki

Kuvaus

Dilute solutions of various dextran standards, a high-molar mass (HMM) commercial dextran from Leuconostoc spp., and HMM dextrans isolated from Weissella confusa and Leuconostoc citreum were analyzed with high-performance size-exclusion chromatography (HPSEC), asymmetric flow field-flow fractionation (AsFlFFF), and diffusion-ordered NMR spectroscopy (DOSY). HPSEC analyses were performed in aqueous and dimethyl sulfoxide (DMSO) solutions, while only aqueous solutions were utilized in AsFlFFF and DOSY. The study showed that all methods were applicable to dextran analysis, but differences between the aqueous and DMSO-based solutions were obtained for HMM samples. These differences were attributed to the presence of aggregates in aqueous solution that were less prevalent in DMSO. The study showed that DOSY provides an estimate of the size of HMM dextrans, though calibration standards may be required for each experimental set-up. To our knowledge, this is the first study utilizing these three methods in analyzing HMM dextrans.

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut199-207
Sivumäärä9
JulkaisuCarbohydrate Polymers
Vuosikerta99
TilaJulkaistu - 1 tammikuuta 2014
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 30819244