Carbon Nanostructure Based Platform for Enzymatic Glutamate Biosensors

Tutkimustuotos: Lehtiartikkeli

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Bibtex - Lataa

@article{e1d61ca7908140829efa10837af0bd96,
title = "Carbon Nanostructure Based Platform for Enzymatic Glutamate Biosensors",
abstract = "Hydrogen peroxide (H2O2) is an important molecule produced in various enzymatic reactions. It is especially important in electrochemical, enzymatic biosensors detecting electroinactive analytes, such as glucose, cholesterol, and glutamate. Thus, there is a strong need for materials that have high affinity for H2O2 oxidation or reduction as well as enable immobilization and sustain enzyme activity without any additional polymer layers. Carbon nanofibers (CNFs) directly grown on tetrahedral amorphous carbon (ta-C) are feasible candidates for this purpose as they possess a reasonably wide water window (1.8 V) and good activity for H2O2 reduction in physiological pH and contain innately large amounts of suitable functional groups for enzyme immobilization. Here we show their use in ultrafast (<0.05 s) detection of H2O2 with the limit of detection of 26 μM and sensitivity of 0.221 A M–1 cm–2. Moreover, we show that ta-C/CNF hybrids can be used directly without the mass-transfer limiting polymer layers as effective immobilization platforms for glutamate oxidase for further applications in ultrafast (<0.05 s) glutamate detection. Finally, rat glial cells cultured on CNFs grown from ta-C without any additional coatings, such as polylysine, showed good adhesion on CNFs and no signs of cytotoxicity, indicating suitability of the material for future in vivo applications. This simplified and miniaturized structure provides an extremely interesting platform for various different enzyme-based electrochemical sensors.",
author = "Noora Isoaho and Emilia Peltola and Sami Sainio and Niklas Wester and Vera Protopopova and Benjamin Wilson and Jari Koskinen and Tomi Laurila",
year = "2017",
doi = "10.1021/acs.jpcc.6b10612",
language = "English",
volume = "121",
pages = "4618–4626",
journal = "Journal of Physical Chemistry C",
issn = "1932-7447",
publisher = "AMERICAN CHEMICAL SOCIETY",
number = "8",

}

RIS - Lataa

TY - JOUR

T1 - Carbon Nanostructure Based Platform for Enzymatic Glutamate Biosensors

AU - Isoaho, Noora

AU - Peltola, Emilia

AU - Sainio, Sami

AU - Wester, Niklas

AU - Protopopova, Vera

AU - Wilson, Benjamin

AU - Koskinen, Jari

AU - Laurila, Tomi

PY - 2017

Y1 - 2017

N2 - Hydrogen peroxide (H2O2) is an important molecule produced in various enzymatic reactions. It is especially important in electrochemical, enzymatic biosensors detecting electroinactive analytes, such as glucose, cholesterol, and glutamate. Thus, there is a strong need for materials that have high affinity for H2O2 oxidation or reduction as well as enable immobilization and sustain enzyme activity without any additional polymer layers. Carbon nanofibers (CNFs) directly grown on tetrahedral amorphous carbon (ta-C) are feasible candidates for this purpose as they possess a reasonably wide water window (1.8 V) and good activity for H2O2 reduction in physiological pH and contain innately large amounts of suitable functional groups for enzyme immobilization. Here we show their use in ultrafast (<0.05 s) detection of H2O2 with the limit of detection of 26 μM and sensitivity of 0.221 A M–1 cm–2. Moreover, we show that ta-C/CNF hybrids can be used directly without the mass-transfer limiting polymer layers as effective immobilization platforms for glutamate oxidase for further applications in ultrafast (<0.05 s) glutamate detection. Finally, rat glial cells cultured on CNFs grown from ta-C without any additional coatings, such as polylysine, showed good adhesion on CNFs and no signs of cytotoxicity, indicating suitability of the material for future in vivo applications. This simplified and miniaturized structure provides an extremely interesting platform for various different enzyme-based electrochemical sensors.

AB - Hydrogen peroxide (H2O2) is an important molecule produced in various enzymatic reactions. It is especially important in electrochemical, enzymatic biosensors detecting electroinactive analytes, such as glucose, cholesterol, and glutamate. Thus, there is a strong need for materials that have high affinity for H2O2 oxidation or reduction as well as enable immobilization and sustain enzyme activity without any additional polymer layers. Carbon nanofibers (CNFs) directly grown on tetrahedral amorphous carbon (ta-C) are feasible candidates for this purpose as they possess a reasonably wide water window (1.8 V) and good activity for H2O2 reduction in physiological pH and contain innately large amounts of suitable functional groups for enzyme immobilization. Here we show their use in ultrafast (<0.05 s) detection of H2O2 with the limit of detection of 26 μM and sensitivity of 0.221 A M–1 cm–2. Moreover, we show that ta-C/CNF hybrids can be used directly without the mass-transfer limiting polymer layers as effective immobilization platforms for glutamate oxidase for further applications in ultrafast (<0.05 s) glutamate detection. Finally, rat glial cells cultured on CNFs grown from ta-C without any additional coatings, such as polylysine, showed good adhesion on CNFs and no signs of cytotoxicity, indicating suitability of the material for future in vivo applications. This simplified and miniaturized structure provides an extremely interesting platform for various different enzyme-based electrochemical sensors.

U2 - 10.1021/acs.jpcc.6b10612

DO - 10.1021/acs.jpcc.6b10612

M3 - Article

VL - 121

SP - 4618

EP - 4626

JO - Journal of Physical Chemistry C

JF - Journal of Physical Chemistry C

SN - 1932-7447

IS - 8

ER -

ID: 10896036