Boar spermatozoa as a biosensor for detecting toxic substances in indoor dust and aerosols

M. A. Andersson, R. Mikkola, S. Rasimus, D. Hoornstra, P. Salin, R. Rahkila, M. Heikkinen, S. Mattila, J. Peltola, S. Kalso, M. Salkinoja-Salonen*

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

Abstrakti

The presence, quantity and origins of potentially toxic airborne substances were searched in moisture damaged indoor environments, where building related ill health symptoms were suspected and reference sites with no health complaints. Boar spermatozoa were used as the toxicity sensor. Indoor aerosols and dusts were collected from kindergartens, schools, offices and residences (n = 25) by electrostatic filtering, vacuuming, wiping from elevated surfaces and from the interior of personal computers. Toxicity was measured from the ethanol or methanol extracts of the dusts and aerosols. EC(50) was expressed as the lowest concentration of the airborne substance that inhibited motility of >50% of the exposed sperm cells compared to vehicle control, within 30 min, 1 day or 3-4 days of exposure. Remarkably toxic aerosols (EC(50)

AlkuperäiskieliEnglanti
Sivut2041-2052
Sivumäärä12
JulkaisuTOXICOLOGY IN VITRO
Vuosikerta24
Numero7
DOI - pysyväislinkit
TilaJulkaistu - lokakuuta 2010
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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