Bioreducible Hydrophobin-Stabilized Supraparticles for Selective Intracellular Release

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

  • Daniele Maiolo
  • Claudia Pigliacelli

  • Paola Sánchez Moreno
  • Martina Bruna Violatto
  • Laura Talamini
  • Ilaria Tirotta
  • Rosanna Piccirillo
  • Massimo Zucchetti
  • Lavinia Morosi
  • Roberta Frapolli
  • Gabriele Candiani
  • Paolo Bigini
  • Pierangelo Metrangolo

  • Francesca Baldelli Bombelli

Organisaatiot

  • Polytechnic University of Milan
  • IRCCS Istituto di ricerche farmacologiche Mario Negri - Milano, Bergamo, Ranica
  • VTT Technical Research Centre of Finland

Kuvaus

One of the main hurdles in nanomedicine is the low stability of drug–nanocarrier complexes as well as the drug delivery efficiency in the region-of-interest. Here, we describe the use of the film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable in vivo and efficiently encapsulate hydrophobic drug molecules. The HFBII film prevents massive release of the encapsulated drug, which, instead, is activated by selective SP disassembly triggered intracellularly by glutathione reduction of the protein film. As a consequence, the therapeutic efficiency of an encapsulated anticancer drug is highly enhanced (2 orders of magnitude decrease in IC50). Biodistribution and pharmacokinetics studies demonstrate the high stability of the loaded SPs in the bloodstream and the selective release of the payloads once taken up in the tissues. Overall, our results provide a rationale for the development of bioreducible and multifunctional nanomedicines.

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut9413-9423
JulkaisuACS Nano
Vuosikerta2017
TilaJulkaistu - 14 elokuuta 2017
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 14856393