Bioconversion of D-xylose to D-xylonate with Kluyveromyces lactis

Yvonne Nygard; Mervi H. Toivari; Merja Penttilae; Laura Ruohonen; Marilyn G. Wiebe

doi:10.1016/j.ymben.2011.04.001

Bioconversion of D-xylose to D-xylonate with Kluyveromyces lactis

Yvonne Nygard^*
, Mervi H. Toivari
, Merja Penttilae
, Laura Ruohonen
, Marilyn G. Wiebe

^*Tämän työn vastaava kirjoittaja

Ei julkaistu Aalto-yliopiston affiliaatiolla

VTT Technical Research Centre of Finland Ltd.

Tutkimustuotos: Lehtiartikkeli › Article › Scientific › vertaisarvioitu

46 Viittaukset (Web of Science)

Abstrakti

D-Xylonate was produced from D-xylose using Kluyveromyces lactis strains which expressed the gene for NADP(+)-dependent D-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19 +/- 2 g D-xylonate l(-1) was produced when K. lactis expressing xyd1 was grown on 10.5 g D-galactose l (1) and 40 g D-xylose l (1). Intracellular accumulation of D-xylonate (up to similar to 70 mg [g biomass](-1)) was observed.

D-Xylose was metabolised to D-xylonate, xylitol and biomass. Oxygen could be reduced to 6 mmol O(2) l(-1) h(-1) without loss in titre or production rate, but metabolism of D-xylose and xylitol were more efficient when 12 mmol O(2) l(-1) h(-1) were provided.

D-Xylose uptake was not affected by deletion of either the D-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1 Delta XYL1 did not produce extracellular xylitol and produced more D-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1 Delta XYL2 produced high concentrations of xylitol and significantly less D-xylonate than the xyd1 strain with the endogenous XYL2. (C) 2011 Elsevier Inc. All rights reserved.

Alkuperäiskieli	Englanti
Sivut	383-391
Sivumäärä	9
Julkaisu	Metabolic Engineering
Vuosikerta	13
Numero	4
DOI - pysyväislinkit	https://doi.org/10.1016/j.ymben.2011.04.001
Tila	Julkaistu - heinäk. 2011
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Rahoitus

Technical assistance of Tarja Laakso, Outi Kononen and Tuuli Teikari is gratefully acknowledged. This study was financially supported by the Academy of Finland through the Centre of Excellence in White Biotechnology-Green Chemistry (grant 118573). The financial support of the European Commission through the Sixth Framework Programme Integrated Project BioSynergy (038994-SES6) is also gratefully acknowledged.

Pääsy asiakirjaan

10.1016/j.ymben.2011.04.001

Siteeraa tätä

@article{41ed1f84169549acaf80e76f06b49176,

title = "Bioconversion of D-xylose to D-xylonate with Kluyveromyces lactis",

abstract = "D-Xylonate was produced from D-xylose using Kluyveromyces lactis strains which expressed the gene for NADP(+)-dependent D-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19 +/- 2 g D-xylonate l(-1) was produced when K. lactis expressing xyd1 was grown on 10.5 g D-galactose l (1) and 40 g D-xylose l (1). Intracellular accumulation of D-xylonate (up to similar to 70 mg [g biomass](-1)) was observed.D-Xylose was metabolised to D-xylonate, xylitol and biomass. Oxygen could be reduced to 6 mmol O(2) l(-1) h(-1) without loss in titre or production rate, but metabolism of D-xylose and xylitol were more efficient when 12 mmol O(2) l(-1) h(-1) were provided.D-Xylose uptake was not affected by deletion of either the D-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1 Delta XYL1 did not produce extracellular xylitol and produced more D-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1 Delta XYL2 produced high concentrations of xylitol and significantly less D-xylonate than the xyd1 strain with the endogenous XYL2. (C) 2011 Elsevier Inc. All rights reserved.",

keywords = "D-xylonic acid, D-xylose, K. lactis, Aeration, D-xylose reductase, Xylitol dehydrogenase, RECOMBINANT SACCHAROMYCES-CEREVISIAE, PSEUDOMONAS-FRAGI, GLUCONOBACTER-OXYDANS, HEXOSE TRANSPORTERS, CARBOXYLIC-ACIDS, YEAST, FERMENTATION, XYLITOL, DEHYDROGENASE, METABOLISM",

author = "Yvonne Nygard and Toivari, \{Mervi H.\} and Merja Penttilae and Laura Ruohonen and Wiebe, \{Marilyn G.\}",

year = "2011",

month = jul,

doi = "10.1016/j.ymben.2011.04.001",

language = "English",

volume = "13",

pages = "383--391",

journal = "Metabolic Engineering",

issn = "1096-7176",

publisher = "Elsevier",

number = "4",

}

TY - JOUR

T1 - Bioconversion of D-xylose to D-xylonate with Kluyveromyces lactis

AU - Nygard, Yvonne

AU - Toivari, Mervi H.

AU - Penttilae, Merja

AU - Ruohonen, Laura

AU - Wiebe, Marilyn G.

PY - 2011/7

Y1 - 2011/7

N2 - D-Xylonate was produced from D-xylose using Kluyveromyces lactis strains which expressed the gene for NADP(+)-dependent D-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19 +/- 2 g D-xylonate l(-1) was produced when K. lactis expressing xyd1 was grown on 10.5 g D-galactose l (1) and 40 g D-xylose l (1). Intracellular accumulation of D-xylonate (up to similar to 70 mg [g biomass](-1)) was observed.D-Xylose was metabolised to D-xylonate, xylitol and biomass. Oxygen could be reduced to 6 mmol O(2) l(-1) h(-1) without loss in titre or production rate, but metabolism of D-xylose and xylitol were more efficient when 12 mmol O(2) l(-1) h(-1) were provided.D-Xylose uptake was not affected by deletion of either the D-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1 Delta XYL1 did not produce extracellular xylitol and produced more D-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1 Delta XYL2 produced high concentrations of xylitol and significantly less D-xylonate than the xyd1 strain with the endogenous XYL2. (C) 2011 Elsevier Inc. All rights reserved.

AB - D-Xylonate was produced from D-xylose using Kluyveromyces lactis strains which expressed the gene for NADP(+)-dependent D-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19 +/- 2 g D-xylonate l(-1) was produced when K. lactis expressing xyd1 was grown on 10.5 g D-galactose l (1) and 40 g D-xylose l (1). Intracellular accumulation of D-xylonate (up to similar to 70 mg [g biomass](-1)) was observed.D-Xylose was metabolised to D-xylonate, xylitol and biomass. Oxygen could be reduced to 6 mmol O(2) l(-1) h(-1) without loss in titre or production rate, but metabolism of D-xylose and xylitol were more efficient when 12 mmol O(2) l(-1) h(-1) were provided.D-Xylose uptake was not affected by deletion of either the D-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1 Delta XYL1 did not produce extracellular xylitol and produced more D-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1 Delta XYL2 produced high concentrations of xylitol and significantly less D-xylonate than the xyd1 strain with the endogenous XYL2. (C) 2011 Elsevier Inc. All rights reserved.

KW - D-xylonic acid

KW - D-xylose

KW - K. lactis

KW - Aeration

KW - D-xylose reductase

KW - Xylitol dehydrogenase

KW - RECOMBINANT SACCHAROMYCES-CEREVISIAE

KW - PSEUDOMONAS-FRAGI

KW - GLUCONOBACTER-OXYDANS

KW - HEXOSE TRANSPORTERS

KW - CARBOXYLIC-ACIDS

KW - YEAST

KW - FERMENTATION

KW - XYLITOL

KW - DEHYDROGENASE

KW - METABOLISM

U2 - 10.1016/j.ymben.2011.04.001

DO - 10.1016/j.ymben.2011.04.001

M3 - Article

SN - 1096-7176

VL - 13

SP - 383

EP - 391

JO - Metabolic Engineering

JF - Metabolic Engineering

IS - 4

ER -

Bioconversion of D-xylose to D-xylonate with Kluyveromyces lactis

Abstrakti

Rahoitus

Pääsy asiakirjaan

Sormenjälki

Siteeraa tätä