Biocatalytic Production of Amino Carbohydrates through Oxidoreductase and Transaminase Cascades

Ville Aumala, Filip Mollerup, Edita Jurak, Fabian Blume, Johanna Karppi, Antti E. Koistinen, Eva Schuiten, Moritz Voß, Uwe Bornscheuer, Jan Deska, Emma R. Master*

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

3 Sitaatiot (Scopus)
157 Lataukset (Pure)

Abstrakti

Plant-derived carbohydrates are an abundant renewable resource. Transformation of carbohydrates into new products, including amine-functionalized building blocks for biomaterials applications, can lower reliance on fossil resources. Herein, biocatalytic production routes to amino carbohydrates, including oligosaccharides, are demonstrated. In each case, two-step biocatalysis was performed to functionalize d-galactose-containing carbohydrates by employing the galactose oxidase from Fusarium graminearum or a pyranose dehydrogenase from Agaricus bisporus followed by the ω-transaminase from Chromobacterium violaceum (Cvi-ω-TA). Formation of 6-amino-6-deoxy-d-galactose, 2-amino-2-deoxy-d-galactose, and 2-amino-2-deoxy-6-aldo-d-galactose was confirmed by mass spectrometry. The activity of Cvi-ω-TA was highest towards 6-aldo-d-galactose, for which the highest yield of 6-amino-6-deoxy-d-galactose (67 %) was achieved in reactions permitting simultaneous oxidation of d-galactose and transamination of the resulting 6-aldo-d-galactose.

AlkuperäiskieliEnglanti
JulkaisuChemSusChem
DOI - pysyväislinkit
TilaJulkaistu - 1 tammikuuta 2019
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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  • Projektit

    Oksidoreduktaasien valjastaminen oligosakkaridien aktivointiin

    Karppi, J., Master, E., Mollerup, F. & Ioannou, E.

    01/09/201731/12/2020

    Projekti: Academy of Finland: Other research funding

    Enzymer som katalysatorer i icke-naturliga syntetiska transformationer

    Blume, F., Jäger, C., Deska, J., Naapuri, J., Liu, Y. & Kiefer, A.

    01/09/201631/03/2021

    Projekti: Academy of Finland: Other research funding

    BHIVE: Bio-derived HIgh Value polymers through novel Enzyme function

    Mollerup, F., Karppi, J., Jurak, E., Aumala, V., Master, E., Monschein, M. & Leamon, A. K. M. A. A.

    01/09/201531/10/2020

    Projekti: EU: ERC grants

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