Asymmetric bioreduction of activated alkenes by a novel isolate of Achromobacter species producing enoate reductase

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

  • Yan Jie Liu
  • Xiao Qiong Pei
  • Hui Lin
  • Ping Gai
  • Yu Chang Liu
  • Zhong Liu Wu

Organisaatiot

  • CAS - Chengdu Institute of Biology
  • University of Chinese Academy of Sciences

Kuvaus

The strain Achromobacter sp. JA81, which produced enoate reductase, was applied in the asymmetric reduction of activated alkenes. The strain could catalyze the bioreduction of alkenes to form enantiopure (R)-ß-aryl- ß-cyano-propanoic acids, a precursor of (R)-?-amino butyric acids, including the pharmaceutically active enantiomer of the chiral drug (R)-baclofen with excellent enantioselectivity. It could catalyze aswell the stereoselective bioreduction of other activated alkenes such as cyclic imides, ß-nitro acrylates, and nitro-alkenes with up to >99 % ee and >99 % conversion. The draft genome sequencing of JA81 revealed six putative old yellow enzyme homologies, and the transcription of one of them, Achr-OYE3, was detected using reverse transcription polymerase chain reaction. The recombinant Escherichia coli expressing Achr-OYE3 displayed enoate reductase activity toward (Z)-3-cyano-3-phenyl-propenoic acid (2a).

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut635-645
Sivumäärä11
JulkaisuApplied Microbiology and Biotechnology
Vuosikerta95
Numero3
TilaJulkaistu - elokuuta 2012
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 14582377