Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor

Surachet Duanghathaipornsuk; Boxuan Shen; Brent D. Cameron; Heini Ijäs; Veikko Linko; Mauri Kostiainen; Dong-Shik Kim

doi:10.1016/j.matlet.2020.128141

Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor

Surachet Duanghathaipornsuk, Boxuan Shen, Brent D. Cameron, Heini Ijäs, Veikko Linko, Mauri Kostiainen, Dong-Shik Kim^*

^*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Lehtiartikkeli › Article › Scientific › vertaisarvioitu

4 Sitaatiot (Scopus)

Abstrakti

DNA origami-based cages functionalized with aptamer motifs were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.

Alkuperäiskieli	Englanti
Artikkeli	128141
Sivumäärä	4
Julkaisu	Materials Letters
Vuosikerta	275
DOI - pysyväislinkit	https://doi.org/10.1016/j.matlet.2020.128141
Tila	Julkaistu - 15 syysk. 2020
OKM-julkaisutyyppi	A1 Julkaistu artikkeli, soviteltu

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10.1016/j.matlet.2020.128141

http://urn.fi/URN:NBN:fi:jyu-202006234420

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SYNVIRO: Polyelektrolyyttien Enkapsulointi Synteettisen Virologian Keinoin (SYNVIRO)
Kostiainen, M., Ahmed, A., Anaya Plaza, E., Liu, Q., Julin, S., Välimäki, S. & Korpi, A.
01/09/2017 → 31/08/2021
Projekti: Academy of Finland: Other research funding
Proteiinihäkeillä suunnattu itsejärjestyminen nanomateriaaleissa
Kostiainen, M., Korpi, A., Damania, A., Liljeström, V. & Ora, A.
01/09/2016 → 31/08/2018
Projekti: Academy of Finland: Other research funding
Räätälöityjä molekyylikoneita nukleiinihappoihin perustuvalla nanoteknologialla
Linko, V.
01/09/2015 → 31/08/2018
Projekti: Academy of Finland: Other research funding

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@article{4a46f04ca8274ad491ebaac01c391f8d,

title = "Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor",

abstract = "DNA origami-based cages functionalized with aptamer motifs were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.",

keywords = "Aptamer, Dissociation constant, DNA origami, Glycated hemoglobin, Surface plasmon resonance sensor",

author = "Surachet Duanghathaipornsuk and Boxuan Shen and Cameron, {Brent D.} and Heini Ij{\"a}s and Veikko Linko and Mauri Kostiainen and Dong-Shik Kim",

year = "2020",

month = sep,

day = "15",

doi = "10.1016/j.matlet.2020.128141",

language = "English",

volume = "275",

journal = "Materials Letters",

issn = "0167-577X",

publisher = "ELSEVIER SCIENCE B.V.",

}

TY - JOUR

T1 - Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor

AU - Duanghathaipornsuk, Surachet

AU - Shen, Boxuan

AU - Cameron, Brent D.

AU - Ijäs, Heini

AU - Linko, Veikko

AU - Kostiainen, Mauri

AU - Kim, Dong-Shik

PY - 2020/9/15

Y1 - 2020/9/15

N2 - DNA origami-based cages functionalized with aptamer motifs were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.

AB - DNA origami-based cages functionalized with aptamer motifs were used to detect hemoglobin and glycated hemoglobin. The binding between the cages and hemoglobin was monitored using a surface plasmon resonance (SPR) sensor. One DNA strand in the nano-cage was replaced with an aptamer that demonstrated a high affinity to hemoglobin (Hb) or glycated hemoglobin (gHb). Three types of the DNA nano-cages designed to fit the size and shape of hemoglobin were evaluated: one without an aptamer, one with the Hb-affinity aptamer (HA) and one with the gHb-affinity aptamer (GHA). Both DNA nano-cages embedded with HA and GHA showed significantly more stable binding with Hb and gHb by 5 and 9 times, respectively, than the aptamers directly immobilized on the SPR surface. HA-embedded DNA and GHA-embedded DNA improved the sensor selectivities by 9 times and 37 times between Hb and gHb.

KW - Aptamer

KW - Dissociation constant

KW - DNA origami

KW - Glycated hemoglobin

KW - Surface plasmon resonance sensor

UR - http://www.scopus.com/inward/record.url?scp=85086640333&partnerID=8YFLogxK

U2 - 10.1016/j.matlet.2020.128141

DO - 10.1016/j.matlet.2020.128141

M3 - Article

VL - 275

JO - Materials Letters

JF - Materials Letters

SN - 0167-577X

M1 - 128141

ER -

Aptamer-embedded DNA origami cage for detecting (glycated) hemoglobin with a surface plasmon resonance sensor

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SYNVIRO: Polyelektrolyyttien Enkapsulointi Synteettisen Virologian Keinoin (SYNVIRO)

Proteiinihäkeillä suunnattu itsejärjestyminen nanomateriaaleissa

Räätälöityjä molekyylikoneita nukleiinihappoihin perustuvalla nanoteknologialla

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