Antibacterial properties of Scots pine and Norway spruce

Tiina Vainio-Kaila

Antibacterial properties of Scots pine and Norway spruce

Julkaisun otsikon käännös: Männyn ja kuusen antibakteeriset ominaisuudet

Tiina Vainio-Kaila

Biotuotteiden ja biotekniikan laitos

Tutkimustuotos: Doctoral Thesis › Collection of Articles

Abstrakti

Puupinnat sisätiloissa vaikuttavat positiivisesti ihmisten terveyteen ja hyvinvointiin. Puun antibakteeriset ominaisuudet saattavat vähentää pintojen kautta tapahtuvan kontaminaation todennäköisyyttä. Antibakteeristen ominaisuuksien parempi ymmärrys mahdollistaa puupintojen hygieenisen laadun paremman hallinnan. Tämän väitöskirjan päätavoite oli selvittää puun ja sen komponenttien antibakteerisia ominaisuuksia. Männyn ja kuusen sydän- ja pintapuun antibakteerisuuden tutkimiseen käytettiin osin tätä työtä varten kehitettyjä menetelmiä. Puupinnan todettiin olevan antibakteerinen myös silloin, kun puun uuteaineet oli poistettu asetonilla. Uutteiden tarkempi tutkimus osoitti erityisesti männyn sydän- ja pintapuun, mutta jonkin verran myös kuusen uutteiden ehkäisevän useiden tautia aiheuttavien bakteereiden mm. metisilliinille resistentin Staphylococcus aureuksen (MRSA) kasvua. Uutteiden lisäksi ligniini oli ainoa erillinen komponentti, jolla todettiin antibakteerisia ominaisuuksia. Puusta haihtuvilla orgaanisilla yhdisteillä (VOC) todettiin antibakteerisia ominaisuuksia useita tautia aiheuttavia bakteerikantoja kohtaan. Kaikissa uutteissa todettiin useita antibakteerisia yhdisteitä, mutta niiden määrä ei aina selittänyt eri puulajien antibakteerisuutta suhteessa toisiin puulajeihin. Mikään yksittäinen yhdiste ei yksin selittänyt antibakteerista vaikutusta, joten ilmiö johtuu joko eri puulajeilla eri mekanismeista tai synergisistä vaikutuksista. α-pineeni ja limoneeni selittivät osin VOCien antibakteerisia ominaisuuksia, mutta myös muilla yhdisteillä todettiin olevan vaikutusta. Puun antibakteerisuuden todettiin johtuvan useista eri aineista ja tehoavan useisiin eri bakteereihin. Nämä tulokset tarjoavat hyvän lähtökohdan puun hygieenisten ominaisuuksien hyödyntämiseen ja niiden kehittämiseen. Uutteet osoittautuivat myös tehokkaiksi ja niiden ominaisuuksia erityisesti suhteessa resistentteihin bakteereihin kannattaisi tutkia lisää.

Julkaisun otsikon käännös	Männyn ja kuusen antibakteeriset ominaisuudet
Alkuperäiskieli	Englanti
Pätevyys	Tohtorintutkinto
Myöntävä instituutio	Aalto-yliopisto
Valvoja/neuvonantaja	Rautkari, Lauri, Vastuuprofessori Hänninen, Tuomas, Ohjaaja Siitonen, Anja, Ohjaaja, Ulkoinen henkilö
Kustantaja	Aalto University
Painoksen ISBN	978-952-60-7621-8
Sähköinen ISBN	978-952-60-7620-1
Tila	Julkaistu - 2017
OKM-julkaisutyyppi	G5 Artikkeliväitöskirja

Tutkimusalat

puun antibakteeriset ominaisuudet
mänty
kuusi

YK:n kestävän kehityksen tavoitteet

Tämä tuotos edistää seuraavia kestävän kehityksen tavoitteita:

Pääsy asiakirjaan

http://urn.fi/URN:ISBN:978-952-60-7620-1Lisenssi: Määrittelemätön

Biotalousinfrastruktuuri
Seppälä, J. (Manager)
Kemian tekniikan korkeakoulu
Laitteistot/tilat: Facility

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title = "Antibacterial properties of Scots pine and Norway spruce",

abstract = "Wooden surfaces in interior use hold potential for improving human health and wellbeing. The antibacterial properties of wood might reduce the possibility of cross-contamination from surfaces. In order to be able to control the hygienic quality of the wooden surface, the antibacterial effect should be better understood. The main aim of this thesis was to identify and evaluate the antibacterial properties of wood and its components. Different methods were developed and used to study the antibacterial properties of Scots pine and Norway spruce, heartwood and sapwood. The solid wood surface showed clear antibacterial properties, even when the extractives had been removed with acetone. Studies with the extracts showed several human pathogens, including methicillin-resistant Staphylococcus aureus, to be susceptible to pine heartwood and sapwood in particular, and also, to some extent, spruce. Besides extractives, lignin was the only separate wood component showing antibacterial properties. Wood volatile organic compounds (VOCs), which were studied in gaseous form, showed an antibacterial effect against various human pathogens. Several antibacterial compounds were found in all the extracts, however, they did not always explain the order of antibacterial activity between wood species. No single compound could alone explain the effect, hence the antibacterial effect derives either from different mechanisms in different species or from a synergistic effect. α-pinene and limonene could partly explain the antibacterial effect of the VOCs, but other components were also found to have an influence. Wood was found to have various antibacterial parts and a diverse range of bacterial pathogens that were sensitive to it. These results offer a good ground for the exploitation of the hygienic properties of wood and a good starting point for enhancing them further. Additionally, the extracts showed promising qualities and they should be studied further in regard to resistant pathogens.",

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year = "2017",

language = "English",

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series = "Aalto University publication series DOCTORAL DISSERTATIONS",

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N2 - Wooden surfaces in interior use hold potential for improving human health and wellbeing. The antibacterial properties of wood might reduce the possibility of cross-contamination from surfaces. In order to be able to control the hygienic quality of the wooden surface, the antibacterial effect should be better understood. The main aim of this thesis was to identify and evaluate the antibacterial properties of wood and its components. Different methods were developed and used to study the antibacterial properties of Scots pine and Norway spruce, heartwood and sapwood. The solid wood surface showed clear antibacterial properties, even when the extractives had been removed with acetone. Studies with the extracts showed several human pathogens, including methicillin-resistant Staphylococcus aureus, to be susceptible to pine heartwood and sapwood in particular, and also, to some extent, spruce. Besides extractives, lignin was the only separate wood component showing antibacterial properties. Wood volatile organic compounds (VOCs), which were studied in gaseous form, showed an antibacterial effect against various human pathogens. Several antibacterial compounds were found in all the extracts, however, they did not always explain the order of antibacterial activity between wood species. No single compound could alone explain the effect, hence the antibacterial effect derives either from different mechanisms in different species or from a synergistic effect. α-pinene and limonene could partly explain the antibacterial effect of the VOCs, but other components were also found to have an influence. Wood was found to have various antibacterial parts and a diverse range of bacterial pathogens that were sensitive to it. These results offer a good ground for the exploitation of the hygienic properties of wood and a good starting point for enhancing them further. Additionally, the extracts showed promising qualities and they should be studied further in regard to resistant pathogens.

AB - Wooden surfaces in interior use hold potential for improving human health and wellbeing. The antibacterial properties of wood might reduce the possibility of cross-contamination from surfaces. In order to be able to control the hygienic quality of the wooden surface, the antibacterial effect should be better understood. The main aim of this thesis was to identify and evaluate the antibacterial properties of wood and its components. Different methods were developed and used to study the antibacterial properties of Scots pine and Norway spruce, heartwood and sapwood. The solid wood surface showed clear antibacterial properties, even when the extractives had been removed with acetone. Studies with the extracts showed several human pathogens, including methicillin-resistant Staphylococcus aureus, to be susceptible to pine heartwood and sapwood in particular, and also, to some extent, spruce. Besides extractives, lignin was the only separate wood component showing antibacterial properties. Wood volatile organic compounds (VOCs), which were studied in gaseous form, showed an antibacterial effect against various human pathogens. Several antibacterial compounds were found in all the extracts, however, they did not always explain the order of antibacterial activity between wood species. No single compound could alone explain the effect, hence the antibacterial effect derives either from different mechanisms in different species or from a synergistic effect. α-pinene and limonene could partly explain the antibacterial effect of the VOCs, but other components were also found to have an influence. Wood was found to have various antibacterial parts and a diverse range of bacterial pathogens that were sensitive to it. These results offer a good ground for the exploitation of the hygienic properties of wood and a good starting point for enhancing them further. Additionally, the extracts showed promising qualities and they should be studied further in regard to resistant pathogens.

KW - antibacterial wood

KW - Scot pine

KW - Norway spruce

KW - puun antibakteeriset ominaisuudet

KW - mänty

KW - kuusi

KW - antibacterial wood

KW - Scot pine

KW - Norway spruce

M3 - Doctoral Thesis

SN - 978-952-60-7621-8

T3 - Aalto University publication series DOCTORAL DISSERTATIONS

PB - Aalto University

ER -

Antibacterial properties of Scots pine and Norway spruce

Abstrakti

Tutkimusalat

YK:n kestävän kehityksen tavoitteet

Pääsy asiakirjaan

Sormenjälki

Laitteet

Biotalousinfrastruktuuri

Siteeraa tätä