An Evaluation of Boar Spermatozoa as a Biosensor for the Detection of Sublethal and Lethal Toxicity

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An Evaluation of Boar Spermatozoa as a Biosensor for the Detection of Sublethal and Lethal Toxicity. / Castagnoli, Emmanuelle; Salo, Johanna; Toivonen, Matti S.; Marik, Tamás; Mikkola, Raimo; Kredics, László; Vicente-Carrillo, Alejandro; Nagy, Szabolcs; Andersson, Markus T.; Andersson, M. A.; Kurnitski, Jarek; Salonen, Heidi.

julkaisussa: Toxins, Vuosikerta 10, Nro 11, 463, 08.11.2018.

Tutkimustuotos: Lehtiartikkeli

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Castagnoli, Emmanuelle ; Salo, Johanna ; Toivonen, Matti S. ; Marik, Tamás ; Mikkola, Raimo ; Kredics, László ; Vicente-Carrillo, Alejandro ; Nagy, Szabolcs ; Andersson, Markus T. ; Andersson, M. A. ; Kurnitski, Jarek ; Salonen, Heidi. / An Evaluation of Boar Spermatozoa as a Biosensor for the Detection of Sublethal and Lethal Toxicity. Julkaisussa: Toxins. 2018 ; Vuosikerta 10, Nro 11.

Bibtex - Lataa

@article{b3a285083f9242408d07a14cbdee4134,
title = "An Evaluation of Boar Spermatozoa as a Biosensor for the Detection of Sublethal and Lethal Toxicity",
abstract = "A novel, objective, and rapid computed motility inhibition (CMI) assay was developed to identify and assess sublethal injury in toxin-exposed boar spermatozoa and compared with a subjective visual motility inhibition (VMI) assay. The CMI values were calculated from digital micrographic videos using a custom MATLAB{\circledR} script by contrasting the motility index values of each experiment with those of the background and control experiments. Following a comparison of the CMI and VMI assays results, it was determined that their agreement depended on the shape of the dose-response curve. Toxins that exhibited a steep slope were indicative of good agreement between the assays. Those depicted by a gentle decline in the slope of the dose-response curve, the CMI assay were shown to be two times more sensitive than the VMI assay. The CMI assay was highly sensitive to the inhibition of mitochondrial function and glucose transport activity by sublethal doses of toxins and to disruption of cellular cation homeostasis by carrier ionophoric toxins, when compared to the cytotoxicity and lethal toxicity assays (i.e., that evaluated the inhibition of cell proliferation in somatic cell lines (FL, PK-15, and MNA cells)) and disruption to spermatozoa membrane integrity. The CMI assay recognized subtle sublethal toxicity changes in metabolism, manifested as a decrease in boar spermatozoa motility. Thus, it was feasible to effectively compare the objectively-measured numerical values for motility inhibition using the CMI assay against those reflecting lethal damage in the spermatozoa cells and somatic cell lines using a cytotoxicity assay.",
keywords = "biological toxins, biosensor, boar spermatozoa, CASA, computed motility inhibition, MATLAB, toxicity",
author = "Emmanuelle Castagnoli and Johanna Salo and Toivonen, {Matti S.} and Tam{\'a}s Marik and Raimo Mikkola and L{\'a}szl{\'o} Kredics and Alejandro Vicente-Carrillo and Szabolcs Nagy and Andersson, {Markus T.} and Andersson, {M. A.} and Jarek Kurnitski and Heidi Salonen",
year = "2018",
month = "11",
day = "8",
doi = "10.3390/toxins10110463",
language = "English",
volume = "10",
journal = "Toxins",
issn = "2072-6651",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "11",

}

RIS - Lataa

TY - JOUR

T1 - An Evaluation of Boar Spermatozoa as a Biosensor for the Detection of Sublethal and Lethal Toxicity

AU - Castagnoli, Emmanuelle

AU - Salo, Johanna

AU - Toivonen, Matti S.

AU - Marik, Tamás

AU - Mikkola, Raimo

AU - Kredics, László

AU - Vicente-Carrillo, Alejandro

AU - Nagy, Szabolcs

AU - Andersson, Markus T.

AU - Andersson, M. A.

AU - Kurnitski, Jarek

AU - Salonen, Heidi

PY - 2018/11/8

Y1 - 2018/11/8

N2 - A novel, objective, and rapid computed motility inhibition (CMI) assay was developed to identify and assess sublethal injury in toxin-exposed boar spermatozoa and compared with a subjective visual motility inhibition (VMI) assay. The CMI values were calculated from digital micrographic videos using a custom MATLAB® script by contrasting the motility index values of each experiment with those of the background and control experiments. Following a comparison of the CMI and VMI assays results, it was determined that their agreement depended on the shape of the dose-response curve. Toxins that exhibited a steep slope were indicative of good agreement between the assays. Those depicted by a gentle decline in the slope of the dose-response curve, the CMI assay were shown to be two times more sensitive than the VMI assay. The CMI assay was highly sensitive to the inhibition of mitochondrial function and glucose transport activity by sublethal doses of toxins and to disruption of cellular cation homeostasis by carrier ionophoric toxins, when compared to the cytotoxicity and lethal toxicity assays (i.e., that evaluated the inhibition of cell proliferation in somatic cell lines (FL, PK-15, and MNA cells)) and disruption to spermatozoa membrane integrity. The CMI assay recognized subtle sublethal toxicity changes in metabolism, manifested as a decrease in boar spermatozoa motility. Thus, it was feasible to effectively compare the objectively-measured numerical values for motility inhibition using the CMI assay against those reflecting lethal damage in the spermatozoa cells and somatic cell lines using a cytotoxicity assay.

AB - A novel, objective, and rapid computed motility inhibition (CMI) assay was developed to identify and assess sublethal injury in toxin-exposed boar spermatozoa and compared with a subjective visual motility inhibition (VMI) assay. The CMI values were calculated from digital micrographic videos using a custom MATLAB® script by contrasting the motility index values of each experiment with those of the background and control experiments. Following a comparison of the CMI and VMI assays results, it was determined that their agreement depended on the shape of the dose-response curve. Toxins that exhibited a steep slope were indicative of good agreement between the assays. Those depicted by a gentle decline in the slope of the dose-response curve, the CMI assay were shown to be two times more sensitive than the VMI assay. The CMI assay was highly sensitive to the inhibition of mitochondrial function and glucose transport activity by sublethal doses of toxins and to disruption of cellular cation homeostasis by carrier ionophoric toxins, when compared to the cytotoxicity and lethal toxicity assays (i.e., that evaluated the inhibition of cell proliferation in somatic cell lines (FL, PK-15, and MNA cells)) and disruption to spermatozoa membrane integrity. The CMI assay recognized subtle sublethal toxicity changes in metabolism, manifested as a decrease in boar spermatozoa motility. Thus, it was feasible to effectively compare the objectively-measured numerical values for motility inhibition using the CMI assay against those reflecting lethal damage in the spermatozoa cells and somatic cell lines using a cytotoxicity assay.

KW - biological toxins

KW - biosensor

KW - boar spermatozoa

KW - CASA

KW - computed motility inhibition

KW - MATLAB

KW - toxicity

UR - http://www.scopus.com/inward/record.url?scp=85056428648&partnerID=8YFLogxK

U2 - 10.3390/toxins10110463

DO - 10.3390/toxins10110463

M3 - Article

VL - 10

JO - Toxins

JF - Toxins

SN - 2072-6651

IS - 11

M1 - 463

ER -

ID: 29800635