Amphipathic design dictates self-assembly, cytotoxicity and cell uptake of arginine-rich surfactant-like peptides

Lucas R. Mello, Rodrigo B. Aguiar, Renata Y. Yamada, Jane Z. Moraes, Ian W. Hamley, Wendel A. Alves, Mehedi Reza, Janne Ruokolainen, Emerson R. Silva*

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

1 Sitaatiot (Scopus)

Abstrakti

Amphiphilicity is the most critical parameter in the self-assembly of surfactant-like peptides (SLPs), regulating the way by which hydrophobic attraction holds peptides together. Its effects go beyond supramolecular assembly and may also trigger different cell responses of bioactive peptide-based nanostructures. Herein, we investigate the self-assembly and cellular effects of nanostructures based on isomeric SLPs composed by arginine (R) and phenylalanine (F). Two amphipathic designs were studied: a diblock construct F4R4 and its bolaamphiphile analog R2F4R2. A strong sequence-dependent polymorphism emerges with appearance of globules and vesicle-like assemblies, or flat nanotapes and cylindrical micelles. The diblock construct possesses good cell penetrating capabilities and effectiveness to kill SK-MEL-28 melanoma tumor cells, in contrast to reduced intracellular uptake and low cytotoxicity exhibited by the bolaamphiphilic form. Our findings demonstrate that amphipathic design is a relevant variable for self-assembling SLPs to modulate different cellular responses and may assist in optimizing the production of nanostructures based on arginine-enriched sequences in cell penetrating and antimicrobial peptides.

AlkuperäiskieliEnglanti
Sivut2495-2507
Sivumäärä13
JulkaisuJournal of Materials Chemistry B
Vuosikerta8
Numero12
DOI - pysyväislinkit
TilaJulkaistu - 28 maaliskuuta 2020
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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  • Siteeraa tätä

    Mello, L. R., Aguiar, R. B., Yamada, R. Y., Moraes, J. Z., Hamley, I. W., Alves, W. A., ... Silva, E. R. (2020). Amphipathic design dictates self-assembly, cytotoxicity and cell uptake of arginine-rich surfactant-like peptides. Journal of Materials Chemistry B, 8(12), 2495-2507. https://doi.org/10.1039/c9tb02219h