Projekteja vuodessa
Abstrakti
We address the problem of de novo design and synthesis of nucleic acid nanostructures, a challenge that has been considered in the area of DNA nanotechnology since the 1980s and more recently in the area of RNA nanotechnology. Toward this goal, we introduce a general algorithmic design process and software pipeline for rendering 3D wireframe polyhedral nanostructures in single-stranded RNA. To initiate the pipeline, the user creates a model of the desired polyhedron using standard 3D graphic design software. As its output, the pipeline produces an RNA nucleotide sequence whose corresponding RNA primary structure can be transcribed from a DNA template and folded in the laboratory. As case examples, we design and characterize experimentally three 3D RNA nanostructures: a tetrahedron, a triangular bipyramid, and a triangular prism. The design software is openly available and also provides an export of the targeted 3D structure into the oxDNA molecular dynamics simulator for easy simulation and visualization.
Alkuperäiskieli | Englanti |
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Sivut | 16608−16616 |
Julkaisu | ACS Nano |
Vuosikerta | 16 |
Numero | 10 |
DOI - pysyväislinkit | |
Tila | Julkaistu - 30 syysk. 2022 |
OKM-julkaisutyyppi | A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä |
Sormenjälki
Sukella tutkimusaiheisiin 'Algorithmic Design of 3D Wireframe RNA Polyhedra'. Ne muodostavat yhdessä ainutlaatuisen sormenjäljen.Projektit
- 2 Päättynyt
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ALBION: Algoritmisia menetelmiä biomolekulaaristen nanorakenteiden suunnitteluun (ALBION)
Orponen, P., Elonen, A., Lohikko, A., Saman Booy, M., Mohammed, A., Vo, T., Gautam, V. & Korpela, D.
01/09/2017 → 31/08/2021
Projekti: Academy of Finland: Other research funding
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DNA-based devices for detection and sensing of biomolecular interactions
Kuzyk, A., Huang, Y., Nguyen, K., Loo, J., Ryssy, J. & Natarajan, A.
01/09/2017 → 31/12/2021
Projekti: Academy of Finland: Other research funding