Adsorption of Proteins on Colloidal Lignin Particles for Advanced Biomaterials

Timo Leskinen*, Joanna Witos, Juan José Valle-Delgado, Kalle Lintinen, Mauri Kostiainen, Susanne K. Wiedmer, Monika Österberg, Maija Liisa Mattinen

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

26 Sitaatiot (Scopus)
180 Lataukset (Pure)

Abstrakti

Coating of colloidal lignin particles (CLPs), or lignin nanoparticles (LNPs), with proteins was evaluated in order to establish a safe, self-assembly mediated modification technique to tune their surface chemistry. Gelatin and poly- l-lysine formed the most pronounced protein corona on the CLP surface, as determined by dynamic light scattering (DLS) and zeta potential measurements. Spherical morphology of individual protein coated CLPs was confirmed by transmission electron (TEM) and atomic force (AFM) microscopy. A mechanistic adsorption study with several random coiled and globular model proteins was carried out using quartz crystal microbalance with dissipation monitoring (QCM-D). The three-dimensional (3D) protein fold structure and certain amino acid interactions were decisive for the protein adsorption on the lignin surface. The main driving forces for protein adsorption were electrostatic, hydrophobic, and van der Waals interactions, and hydrogen bonding. The relative contributions of these interactions were highly dependent on the ionic strength of the surrounding medium. Capillary electrophoresis (CE) and Fourier transform infrared spectroscopy (FTIR) provided further evidence of the adsorption-enhancing role of specific amino acid residues such as serine and proline. These results have high impact on the utilization of lignin as colloidal particles in biomedicine and biodegradable materials, as the protein corona enables tailoring of the CLP surface chemistry for intended applications.

AlkuperäiskieliEnglanti
Sivut2767-2776
Sivumäärä10
JulkaisuBiomacromolecules
Vuosikerta18
Numero9
DOI - pysyväislinkit
TilaJulkaistu - 11 syyskuuta 2017
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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  • Projektit

    ZELCOR: Zero Waste Ligno-Cellulosic Biorefineries by Integrated Lignin Valorisation

    Österberg, M.

    01/10/201631/12/2020

    Projekti: EU: Framework programmes funding

    TaBioMat: Biomassasta räätätöidyt itsestään järjestäytyvät rakennuspalikat bionanomateriaaleihin

    Mattinen, M., Johansson, L., Zhang, X., Farooq, M., Riviere, G., Zou, T. & Leskinen, T.

    01/09/201521/09/2018

    Projekti: Academy of Finland: Other research funding

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