Acrebol, a novel toxic peptaibol produced by an Acremonium exuviarum indoor isolate

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Acrebol, a novel toxic peptaibol produced by an Acremonium exuviarum indoor isolate. / Andersson, M. A.; Mikkola, R.; Raulio, M.; Kredics, L.; Maijala, P.; Salkinoja-Salonen, M. S.

julkaisussa: Journal of Applied Microbiology, Vuosikerta 106, Nro 3, 03.2009, s. 909-923.

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

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Andersson, M. A. ; Mikkola, R. ; Raulio, M. ; Kredics, L. ; Maijala, P. ; Salkinoja-Salonen, M. S. / Acrebol, a novel toxic peptaibol produced by an Acremonium exuviarum indoor isolate. Julkaisussa: Journal of Applied Microbiology. 2009 ; Vuosikerta 106, Nro 3. Sivut 909-923.

Bibtex - Lataa

@article{a6a5cad496e14eeb987b325c5f819a34,
title = "Acrebol, a novel toxic peptaibol produced by an Acremonium exuviarum indoor isolate",
abstract = "To identify a toxin and its producer isolated from woody material in a building where the occupants experienced serious ill health symptoms.Hyphal extracts of an indoor fungus, identified as the cycloheximide-tolerant species Acremonium exuviarum, inhibited motility of boar spermatozoa (EC(50) 5 +/- 2 mu g of crude solids ml(-1)) and caused cytolysis of murine neuroblastoma cells (MNA) and feline fetal lung cells (FL). The responsible substances were purified and identified as two structurally similar, heat-stable, novel, toxic peptaibols, 1726 Da and 1740 Da, respectively, with amino acid sequences of Acetyl-Phe-Iva/Val-Gln-Aib-Ile-Thr-Leu-Aib-Pro-Aib-Gln-Pro-Aib-(X-X-X)-SerOH and Acetyl-Phe-Iva/Val-Gln-Aib-Ile-Thr-Leu-Val-Pro-Aib-Gln-Pro-Aib-(X-X-X)-SerOH. Purified acrebol inhibited motility of boar sperm, depleted ATP half-content in 1 day (EC(50) of 0.1 mu g ml(-1), 60 nmol l(-1)) depolarised the mitochondria after 2 days, but did not affect the cellular content in NADH. This indicates mitochondrial toxicity. Plate-grown biomass of A. exuviarum BMB4 contained 0.1-1{\%} (w/w) of acrebol, depending on the culture medium.Acrebol paralysed the energy generation of mammalian cells suggesting that mitochondria were its target of action.Acremonium exuviarum, as an indoor fungus, is potentially hazardous to health because of the toxic peptaibols that it produces.",
keywords = "acrebol, antimycin, mitochondrial toxicity, moldy building, myxothiazol, sperm toxic, BUILDING-MATERIALS, DAMAGED BUILDINGS, MAMMALIAN-CELLS, MOLD GROWTH, ION-CHANNEL, SP-NOV, STACHYBOTRYS, ENVIRONMENT, PEPTAIBIOTICS, ANTIBIOTICS",
author = "Andersson, {M. A.} and R. Mikkola and M. Raulio and L. Kredics and P. Maijala and Salkinoja-Salonen, {M. S.}",
year = "2009",
month = "3",
doi = "10.1111/j.1365-2672.2008.04062.x",
language = "English",
volume = "106",
pages = "909--923",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley",
number = "3",

}

RIS - Lataa

TY - JOUR

T1 - Acrebol, a novel toxic peptaibol produced by an Acremonium exuviarum indoor isolate

AU - Andersson, M. A.

AU - Mikkola, R.

AU - Raulio, M.

AU - Kredics, L.

AU - Maijala, P.

AU - Salkinoja-Salonen, M. S.

PY - 2009/3

Y1 - 2009/3

N2 - To identify a toxin and its producer isolated from woody material in a building where the occupants experienced serious ill health symptoms.Hyphal extracts of an indoor fungus, identified as the cycloheximide-tolerant species Acremonium exuviarum, inhibited motility of boar spermatozoa (EC(50) 5 +/- 2 mu g of crude solids ml(-1)) and caused cytolysis of murine neuroblastoma cells (MNA) and feline fetal lung cells (FL). The responsible substances were purified and identified as two structurally similar, heat-stable, novel, toxic peptaibols, 1726 Da and 1740 Da, respectively, with amino acid sequences of Acetyl-Phe-Iva/Val-Gln-Aib-Ile-Thr-Leu-Aib-Pro-Aib-Gln-Pro-Aib-(X-X-X)-SerOH and Acetyl-Phe-Iva/Val-Gln-Aib-Ile-Thr-Leu-Val-Pro-Aib-Gln-Pro-Aib-(X-X-X)-SerOH. Purified acrebol inhibited motility of boar sperm, depleted ATP half-content in 1 day (EC(50) of 0.1 mu g ml(-1), 60 nmol l(-1)) depolarised the mitochondria after 2 days, but did not affect the cellular content in NADH. This indicates mitochondrial toxicity. Plate-grown biomass of A. exuviarum BMB4 contained 0.1-1% (w/w) of acrebol, depending on the culture medium.Acrebol paralysed the energy generation of mammalian cells suggesting that mitochondria were its target of action.Acremonium exuviarum, as an indoor fungus, is potentially hazardous to health because of the toxic peptaibols that it produces.

AB - To identify a toxin and its producer isolated from woody material in a building where the occupants experienced serious ill health symptoms.Hyphal extracts of an indoor fungus, identified as the cycloheximide-tolerant species Acremonium exuviarum, inhibited motility of boar spermatozoa (EC(50) 5 +/- 2 mu g of crude solids ml(-1)) and caused cytolysis of murine neuroblastoma cells (MNA) and feline fetal lung cells (FL). The responsible substances were purified and identified as two structurally similar, heat-stable, novel, toxic peptaibols, 1726 Da and 1740 Da, respectively, with amino acid sequences of Acetyl-Phe-Iva/Val-Gln-Aib-Ile-Thr-Leu-Aib-Pro-Aib-Gln-Pro-Aib-(X-X-X)-SerOH and Acetyl-Phe-Iva/Val-Gln-Aib-Ile-Thr-Leu-Val-Pro-Aib-Gln-Pro-Aib-(X-X-X)-SerOH. Purified acrebol inhibited motility of boar sperm, depleted ATP half-content in 1 day (EC(50) of 0.1 mu g ml(-1), 60 nmol l(-1)) depolarised the mitochondria after 2 days, but did not affect the cellular content in NADH. This indicates mitochondrial toxicity. Plate-grown biomass of A. exuviarum BMB4 contained 0.1-1% (w/w) of acrebol, depending on the culture medium.Acrebol paralysed the energy generation of mammalian cells suggesting that mitochondria were its target of action.Acremonium exuviarum, as an indoor fungus, is potentially hazardous to health because of the toxic peptaibols that it produces.

KW - acrebol

KW - antimycin

KW - mitochondrial toxicity

KW - moldy building

KW - myxothiazol

KW - sperm toxic

KW - BUILDING-MATERIALS

KW - DAMAGED BUILDINGS

KW - MAMMALIAN-CELLS

KW - MOLD GROWTH

KW - ION-CHANNEL

KW - SP-NOV

KW - STACHYBOTRYS

KW - ENVIRONMENT

KW - PEPTAIBIOTICS

KW - ANTIBIOTICS

U2 - 10.1111/j.1365-2672.2008.04062.x

DO - 10.1111/j.1365-2672.2008.04062.x

M3 - Article

VL - 106

SP - 909

EP - 923

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 3

ER -

ID: 7090534