Acrebol, a novel toxic peptaibol produced by an Acremonium exuviarum indoor isolate

Tutkimustuotos: Lehtiartikkelivertaisarvioitu

Tutkijat

Organisaatiot

  • University of Helsinki
  • University of Szeged

Kuvaus

To identify a toxin and its producer isolated from woody material in a building where the occupants experienced serious ill health symptoms.

Hyphal extracts of an indoor fungus, identified as the cycloheximide-tolerant species Acremonium exuviarum, inhibited motility of boar spermatozoa (EC(50) 5 +/- 2 mu g of crude solids ml(-1)) and caused cytolysis of murine neuroblastoma cells (MNA) and feline fetal lung cells (FL). The responsible substances were purified and identified as two structurally similar, heat-stable, novel, toxic peptaibols, 1726 Da and 1740 Da, respectively, with amino acid sequences of Acetyl-Phe-Iva/Val-Gln-Aib-Ile-Thr-Leu-Aib-Pro-Aib-Gln-Pro-Aib-(X-X-X)-SerOH and Acetyl-Phe-Iva/Val-Gln-Aib-Ile-Thr-Leu-Val-Pro-Aib-Gln-Pro-Aib-(X-X-X)-SerOH. Purified acrebol inhibited motility of boar sperm, depleted ATP half-content in 1 day (EC(50) of 0.1 mu g ml(-1), 60 nmol l(-1)) depolarised the mitochondria after 2 days, but did not affect the cellular content in NADH. This indicates mitochondrial toxicity. Plate-grown biomass of A. exuviarum BMB4 contained 0.1-1% (w/w) of acrebol, depending on the culture medium.

Acrebol paralysed the energy generation of mammalian cells suggesting that mitochondria were its target of action.

Acremonium exuviarum, as an indoor fungus, is potentially hazardous to health because of the toxic peptaibols that it produces.

Yksityiskohdat

AlkuperäiskieliEnglanti
Sivut909-923
Sivumäärä15
JulkaisuJournal of Applied Microbiology
Vuosikerta106
Numero3
TilaJulkaistu - maaliskuuta 2009
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

ID: 7090534