A self-assembling fluorescent dipeptide conjugate for cell labelling

Steven Kirkham; Ian W. Hamley; Andrew M. Smith; Ricardo M. Gouveia; Che J. Connon; Mehedi Reza; Janne Ruokolainen

doi:10.1016/j.colsurfb.2015.04.062

A self-assembling fluorescent dipeptide conjugate for cell labelling

Steven Kirkham
, Ian W. Hamley^*
, Andrew M. Smith
, Ricardo M. Gouveia
, Che J. Connon
, Mehedi Reza
, Janne Ruokolainen

^*Tämän työn vastaava kirjoittaja

University of Reading
University of Newcastle upon Tyne

Tutkimustuotos: Lehtiartikkeli › Article › Scientific › vertaisarvioitu

17 Sitaatiot (Scopus)

330 Lataukset (Pure)

Abstrakti

Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).

Alkuperäiskieli	Englanti
Sivut	104-108
Sivumäärä	5
Julkaisu	Colloids and Surfaces B: Biointerfaces
Vuosikerta	137
Numero	1 Jan 2016
DOI - pysyväislinkit	https://doi.org/10.1016/j.colsurfb.2015.04.062
Tila	Julkaistu - 1 tammik. 2016
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Rahoitus

This research was supported by BBSRC grant BB/I008187/1 (CJC/IWH) and EPSRC grant EP/L020599/1 to IWH. We are grateful to Katsuaki Inoue and James Doutch for assistance at Diamond (beamtime reference SM10007), to Emerson da Silva for the analysis of the cac data, and to Ashkan Dehsorkhi for assistance with SAXS measurements.

Pääsy asiakirjaan

10.1016/j.colsurfb.2015.04.062

1-s2.0-S0927776515002817-mainFinal published version, 1,16 MBLisenssi: CC BY

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http://www.sciencedirect.com/science/article/pii/S0927776515002817

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title = "A self-assembling fluorescent dipeptide conjugate for cell labelling",

abstract = "Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).",

keywords = "Peptides, Peptide conjugates, Self-assembly, Fluorescence, SUPRAMOLECULAR HYDROGELS, ENZYMATIC FORMATION, SMALL MOLECULES, PEPTIDE, NANOPARTICLES, NANOFIBERS/HYDROGELS, CYTOTOXICITY, DERIVATIVES, DELIVERY, ENHANCE",

author = "Steven Kirkham and Hamley, \{Ian W.\} and Smith, \{Andrew M.\} and Gouveia, \{Ricardo M.\} and Connon, \{Che J.\} and Mehedi Reza and Janne Ruokolainen",

year = "2016",

month = jan,

day = "1",

doi = "10.1016/j.colsurfb.2015.04.062",

language = "English",

volume = "137",

pages = "104--108",

journal = "Colloids and Surfaces B: Biointerfaces",

issn = "0927-7765",

publisher = "Elsevier",

number = "1 Jan 2016",

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TY - JOUR

T1 - A self-assembling fluorescent dipeptide conjugate for cell labelling

AU - Kirkham, Steven

AU - Hamley, Ian W.

AU - Smith, Andrew M.

AU - Gouveia, Ricardo M.

AU - Connon, Che J.

AU - Reza, Mehedi

AU - Ruokolainen, Janne

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).

AB - Derivatives of fluorophore FITC (fluorescein isothiocyanate) are widely used in bioassays to label proteins and cells. An N-terminal leucine dipeptide is attached to FITC, and we show that this simple conjugate molecule is cytocompatible and is uptaken by cells (human dermal and corneal fibroblasts) in contrast to FITC itself. Co-localisation shows that FITC-LL segregates in pen-nuclear and intracellular vesicle regions. Above a critical aggregation concentration, the conjugate is shown to self-assemble into beta-sheet nanostructures comprising molecular bilayers. (C) 2015 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.orgflicenses/by/4.0/).

KW - Peptides

KW - Peptide conjugates

KW - Self-assembly

KW - Fluorescence

KW - SUPRAMOLECULAR HYDROGELS

KW - ENZYMATIC FORMATION

KW - SMALL MOLECULES

KW - PEPTIDE

KW - NANOPARTICLES

KW - NANOFIBERS/HYDROGELS

KW - CYTOTOXICITY

KW - DERIVATIVES

KW - DELIVERY

KW - ENHANCE

UR - http://www.sciencedirect.com/science/article/pii/S0927776515002817

U2 - 10.1016/j.colsurfb.2015.04.062

DO - 10.1016/j.colsurfb.2015.04.062

M3 - Article

SN - 0927-7765

VL - 137

SP - 104

EP - 108

JO - Colloids and Surfaces B: Biointerfaces

JF - Colloids and Surfaces B: Biointerfaces

IS - 1 Jan 2016

ER -

A self-assembling fluorescent dipeptide conjugate for cell labelling

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