A label-free light-scattering method to resolve assembly and disassembly of DNA nanostructures

Heini Ijäs, Tim Liedl, Veikko Linko*, Gregor Posnjak

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArticleScientificvertaisarvioitu

1 Sitaatiot (Scopus)
11 Lataukset (Pure)


DNA self-assembly, and in particular DNA origami, has evolved into a reliable workhorse for organizing organic and inorganic materials with nanometer precision and with exactly controlled stoichiometry. To ensure the intended performance of a given DNA structure, it is beneficial to determine its folding temperature, which in turn yields the best possible assembly of all DNA strands. Here, we show that temperature-controlled sample holders and standard fluorescence spectrometers or dynamic light-scattering setups in a static light-scattering configuration allow for monitoring the assembly progress in real time. With this robust label-free technique, we determine the folding and melting temperatures of a set of different DNA origami structures without the need for more tedious protocols. In addition, we use the method to follow digestion of DNA structures in the presence of DNase I and find strikingly different resistances toward enzymatic degradation depending on the structural design of the DNA object.

JulkaisuBiophysical Journal
DOI - pysyväislinkit
TilaJulkaistu - 20 jouluk. 2022
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu


Sukella tutkimusaiheisiin 'A label-free light-scattering method to resolve assembly and disassembly of DNA nanostructures'. Ne muodostavat yhdessä ainutlaatuisen sormenjäljen.

Siteeraa tätä