3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks

Hossein Bani Asadi; Roberta Teixeira Polez; Erfan Kimiaei; Zahraalsadat Madani; Orlando Rojas Gaona; Monika Österberg; Jukka Seppälä

doi:10.1016/j.ijbiomac.2021.10.078

3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks

Hossein Bani Asadi, Roberta Teixeira Polez, Erfan Kimiaei, Zahraalsadat Madani, Orlando Rojas Gaona, Monika Österberg, Jukka Seppälä^*

^*Tämän työn vastaava kirjoittaja

University of British Columbia

Tutkimustuotos: Lehtiartikkeli › Article › Scientific › vertaisarvioitu

120 Lataukset (Pure)

Abstrakti

Plant-based hydrogels have attracted great attention in biomedical fields since they are biocompatible and based on natural, sustainable, cost-effective, and widely accessible sources. Here, we introduced new viscoelastic bio-inks composed of quince seed mucilage and cellulose nanofibrils (QSM/CNF) easily extruded into 3D lattice structures through direct ink writing in ambient conditions. The QSM/CNF inks enabled precise control on printing fidelity where CNF endowed objects with shape stability after freeze-drying and with suitable porosity, water uptake capacity, and mechanical strength. The compressive and elastic moduli of samples produced at the highest CNF content were both increased by ~100% (from 5.1 ± 0.2 kPa and 32 ± 1 kPa to 10.7 ± 0.5 and 64 ± 2 kPa, respectively). These values ideally matched those reported for soft tissues; accordingly, the cell compatibility of the printed samples was evaluated against HepG2 cells (human liver cancer). The results confirmed the 3D hydrogels as being non-cytotoxic and suitable to support attachment, survival, and proliferation of the cells. All in all, the newly developed inks allowed sustainable 3D bio-hydrogels fitting the requirements as scaffolds for soft tissue engineering.

Alkuperäiskieli	Englanti
Sivut	1098-1107
Sivumäärä	10
Julkaisu	International Journal of Biological Macromolecules
Vuosikerta	192
Varhainen verkossa julkaisun päivämäärä	16 lokak. 2021
DOI - pysyväislinkit	https://doi.org/10.1016/j.ijbiomac.2021.10.078
Tila	Julkaistu - 1 jouluk. 2021
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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10.1016/j.ijbiomac.2021.10.078Lisenssi: CC BY

3D printing and properties of cellulose nanofibrils-reinforced quince seedFinal published version, 4,63 MBLisenssi: CC BY

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2 Päättynyt

BIOECONOMY: BIOECONOMY Alliance for excellence in sustainable biomass refining
Linder, M.
01/01/2020 → 31/12/2023
Projekti: Academy of Finland: Other research funding
VALUEBIOMAT: Bio-öljyihin perustuvat polymeerikomposiitit; arvoketju synteesistä digitaaliseen valmistukseen
Seppälä, J., Äkräs, L., Revitzer, H., van Bochove, B., Bani Asadi, H., Farzan, A., Dienel, K., Teotia, A., Borandeh, S. & Madani, M.
01/06/2019 → 28/02/2023
Projekti: Academy of Finland: Strategic research funding
FinnCERES: Competence Center for the Materials Bioeconomy: A Flagship for our Sustainable Future
Mäkelä, K.
01/05/2018 → 31/12/2022
Projekti: Academy of Finland: Other research funding

Biotalousinfrastruktuuri
Jukka Seppälä (Manager)
Kemian tekniikan korkeakoulu
Laitteistot/tilat: Facility

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title = "3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks",

abstract = "Plant-based hydrogels have attracted great attention in biomedical fields since they are biocompatible and based on natural, sustainable, cost-effective, and widely accessible sources. Here, we introduced new viscoelastic bio-inks composed of quince seed mucilage and cellulose nanofibrils (QSM/CNF) easily extruded into 3D lattice structures through direct ink writing in ambient conditions. The QSM/CNF inks enabled precise control on printing fidelity where CNF endowed objects with shape stability after freeze-drying and with suitable porosity, water uptake capacity, and mechanical strength. The compressive and elastic moduli of samples produced at the highest CNF content were both increased by ~100% (from 5.1 ± 0.2 kPa and 32 ± 1 kPa to 10.7 ± 0.5 and 64 ± 2 kPa, respectively). These values ideally matched those reported for soft tissues; accordingly, the cell compatibility of the printed samples was evaluated against HepG2 cells (human liver cancer). The results confirmed the 3D hydrogels as being non-cytotoxic and suitable to support attachment, survival, and proliferation of the cells. All in all, the newly developed inks allowed sustainable 3D bio-hydrogels fitting the requirements as scaffolds for soft tissue engineering.",

author = "{Bani Asadi}, Hossein and {Teixeira Polez}, Roberta and Erfan Kimiaei and Zahraalsadat Madani and {Rojas Gaona}, Orlando and Monika {\"O}sterberg and Jukka Sepp{\"a}l{\"a}",

note = "The authors would like to acknowledge the Academy of Finland funding; No. 327248 (ValueBiomat) and 327865 (Bioeconomy). This work was a part of the Academy of Finland's Flagship Programme under Projects No. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). The authors would also like to thank Ms. Marja K{\"a}rkk{\"a}inen for providing TEMPO-CNF and the Biohybrid Materials Research Group (Aalto University) for providing the HepG2 cells.",

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doi = "10.1016/j.ijbiomac.2021.10.078",

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journal = "International Journal of Biological Macromolecules",

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T1 - 3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks

AU - Bani Asadi, Hossein

AU - Teixeira Polez, Roberta

AU - Kimiaei, Erfan

AU - Madani, Zahraalsadat

AU - Rojas Gaona, Orlando

AU - Österberg, Monika

AU - Seppälä, Jukka

N1 - The authors would like to acknowledge the Academy of Finland funding; No. 327248 (ValueBiomat) and 327865 (Bioeconomy). This work was a part of the Academy of Finland's Flagship Programme under Projects No. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). The authors would also like to thank Ms. Marja Kärkkäinen for providing TEMPO-CNF and the Biohybrid Materials Research Group (Aalto University) for providing the HepG2 cells.

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Plant-based hydrogels have attracted great attention in biomedical fields since they are biocompatible and based on natural, sustainable, cost-effective, and widely accessible sources. Here, we introduced new viscoelastic bio-inks composed of quince seed mucilage and cellulose nanofibrils (QSM/CNF) easily extruded into 3D lattice structures through direct ink writing in ambient conditions. The QSM/CNF inks enabled precise control on printing fidelity where CNF endowed objects with shape stability after freeze-drying and with suitable porosity, water uptake capacity, and mechanical strength. The compressive and elastic moduli of samples produced at the highest CNF content were both increased by ~100% (from 5.1 ± 0.2 kPa and 32 ± 1 kPa to 10.7 ± 0.5 and 64 ± 2 kPa, respectively). These values ideally matched those reported for soft tissues; accordingly, the cell compatibility of the printed samples was evaluated against HepG2 cells (human liver cancer). The results confirmed the 3D hydrogels as being non-cytotoxic and suitable to support attachment, survival, and proliferation of the cells. All in all, the newly developed inks allowed sustainable 3D bio-hydrogels fitting the requirements as scaffolds for soft tissue engineering.

AB - Plant-based hydrogels have attracted great attention in biomedical fields since they are biocompatible and based on natural, sustainable, cost-effective, and widely accessible sources. Here, we introduced new viscoelastic bio-inks composed of quince seed mucilage and cellulose nanofibrils (QSM/CNF) easily extruded into 3D lattice structures through direct ink writing in ambient conditions. The QSM/CNF inks enabled precise control on printing fidelity where CNF endowed objects with shape stability after freeze-drying and with suitable porosity, water uptake capacity, and mechanical strength. The compressive and elastic moduli of samples produced at the highest CNF content were both increased by ~100% (from 5.1 ± 0.2 kPa and 32 ± 1 kPa to 10.7 ± 0.5 and 64 ± 2 kPa, respectively). These values ideally matched those reported for soft tissues; accordingly, the cell compatibility of the printed samples was evaluated against HepG2 cells (human liver cancer). The results confirmed the 3D hydrogels as being non-cytotoxic and suitable to support attachment, survival, and proliferation of the cells. All in all, the newly developed inks allowed sustainable 3D bio-hydrogels fitting the requirements as scaffolds for soft tissue engineering.

U2 - 10.1016/j.ijbiomac.2021.10.078

DO - 10.1016/j.ijbiomac.2021.10.078

M3 - Article

SN - 0141-8130

VL - 192

SP - 1098

EP - 1107

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks

Abstrakti

YK:n kestävän kehityksen tavoitteet

Pääsy asiakirjaan

OpenUrl-saatavuus

Sormenjälki

Projektit

BIOECONOMY: BIOECONOMY Alliance for excellence in sustainable biomass refining

VALUEBIOMAT: Bio-öljyihin perustuvat polymeerikomposiitit; arvoketju synteesistä digitaaliseen valmistukseen

FinnCERES: Competence Center for the Materials Bioeconomy: A Flagship for our Sustainable Future

Laitteet

Biotalousinfrastruktuuri

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