Dual-action host-guest chemistry suppresses PAO1 virulence in A549 cell line model

Organism: Homo sapiens
Experiment type: Expression profiling by high throughput sequencing
Summary Our study showed that selected pillararene was able to decrease the level of cell death and demage caused to human A549 epithelial cells by Pseudomonas aeruginosa PAO1 infection. To further understand the protective effects of pillararene during bacterial infections, we analyzed total mRNA isolated from the A549 epithelial cells. Our study showed that pillararene alone had minimal effect on A549 epithelial cells. The addition of PAO1 to A549 cells greatly altered expression levels. The addition of pillararene to infected A549 epithelial cells displayed a concentration-dependent reduction in the inflammatory response.

Overall design: Human epithelial lung cells (A549)were grown in the presence and absence of Pseudomonas aeruginosa PAO1 and two different concentrations of pillararene (0.1 and 0.5 mM).

The raw data files for each sample in this submission are not de-multiplexed. All sample metadata are included in the file sample_information.xls linked to to the foot of this record.