Yeast strains producing cellulolytic enzymes and methods and means for constructing them.

JONATHAN KNOWLES, (Inventor), MERJA PENTTILA, (Inventor), TUULA TEERI, (Inventor), HELENA NEVALAINEN, (Inventor), IRMA SALOVUORI, (Inventor), PAIVI LEHTOVAARA-HELENIUS, (Inventor)

Research output: Patent

Abstract

This is achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze beta -1,4-glucan substrates such as cellulose.

Original languageEnglish
Patent numberEP0312121
IPCC12N 15/ 81 A I
Priority date13/04/1984
Publication statusPublished - 19 Apr 1989
MoE publication typeH1 Granted patent

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