Wood Extractives Promote Cellulase Activity on Cellulosic Substrates

Timo Leskinen, Carlos Salas, Stephen S. Kelley, Dimitris S. Argyropoulos*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

Deposition of hydrophobic wood extractives and representative model compounds, on the surface of cellulose prior to enzymatic hydrolysis was found to either enhance or inhibit the action of cellulase enzymes. The effect of these compounds was correlated with their chemical structure, which may in part explain the differential effects observed between softwood and hardwood extractives. Specifically, the addition of sterol, enhanced enzymatic hydrolysis of microcrystalline cellulose by 54%, whereas the addition of a triglyceride could inhibit the hydrolysis by 49%. The effects of the different extractive's could be explained by considering their Hansen solubility parameters. The amphiphilic and/or hydrophobic character of model extractives was found to be the variable that affected the deposition of extractives on cellulose surfaces and the eventual adsorption of cellulolytic enzymes on it. The observed beneficial effects of extractives are likely related to a reduction in the irreversible binding of the enzymes on the cellulose surface.

Original languageEnglish
Pages (from-to)3226-3234
Number of pages9
JournalBiomacromolecules
Volume16
Issue number10
DOIs
Publication statusPublished - 12 Oct 2015
MoE publication typeA1 Journal article-refereed

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    Leskinen, T., Salas, C., Kelley, S. S., & Argyropoulos, D. S. (2015). Wood Extractives Promote Cellulase Activity on Cellulosic Substrates. Biomacromolecules, 16(10), 3226-3234. https://doi.org/10.1021/acs.biomac.5b00889