Use of a modified alcohol dehydrogenase, ADH1, promoter in construction of diacetyl non-producing brewer's yeast

Maija-Leena Onnela, Maija-Liisa Suihko, Sirkka Keränen, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

The bacterial gene, encoding alpha-acetolactate decarboxylase (alpha-ALDC), was expressed in a bottom-fermenting brewer's yeast under the control of a modified Saccharomyces cerevisiae alcohol dehydrogenase (ADHI) promoter which lacks the upstream regions from -800 bp to -1500 bp. In pilot scale brewing conditions, the level of alpha-ALDC produced was high enough to reduce the concentration of diacetyl so that lagering was not required. alpha-ALDC active brewer's yeast strains were also shown to be suitable for high gravity brewing.

Original languageEnglish
Pages (from-to)101-109
Number of pages9
JournalJournal of Biotechnology
Volume49
Issue number1-3
DOIs
Publication statusPublished - 20 Aug 1996
MoE publication typeA1 Journal article-refereed

Keywords

  • brewer's yeast
  • alpha-acetolactate decarboxylase
  • ADH1 promoter
  • promoter
  • ALPHA-ACETOLACTATE DECARBOXYLASE
  • SACCHAROMYCES-CEREVISIAE
  • ENTEROBACTER-AEROGENES
  • GENE
  • EXPRESSION
  • CLONING
  • STRAINS
  • XYLINUM

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