TY - JOUR
T1 - Two-step nitrification in a pure moving bed biofilm reactor-membrane bioreactor for wastewater treatment
T2 - nitrifying and denitrifying microbial populations and kinetic modeling
AU - Leyva-Díaz, J. C.
AU - González-Martínez, A.
AU - Muñío, M. M.
AU - Poyatos, J. M.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The moving bed biofilm reactor-membrane bioreactor (MBBR-MBR) is a novel solution to conventional activated sludge processes and membrane bioreactors. In this study, a pure MBBR-MBR was studied. The pure MBBR-MBR mainly had attached biomass. The bioreactor operated with a hydraulic retention time (HRT) of 9.5 h. The kinetic parameters for heterotrophic and autotrophic biomasses, mainly nitrite-oxidizing bacteria (NOB), were evaluated. The analysis of the bacterial community structure of the ammonium-oxidizing bacteria (AOB), NOB, and denitrifying bacteria (DeNB) from the pure MBBR-MBR was carried out by means of pyrosequencing to detect and quantify the contribution of the nitrifying and denitrifying bacteria in the total bacterial community. The relative abundance of AOB, NOB, and DeNB were 5, 1, and 3 %, respectively, in the mixed liquor suspended solids (MLSS), and these percentages were 18, 5, and 2 %, respectively, in the biofilm density (BD) attached to carriers. The pure MBBR-MBR had a high efficiency of total nitrogen (TN) removal of 71.81 ± 16.04 %, which could reside in the different bacterial assemblages in the fixed biofilm on the carriers. In this regard, the kinetic parameters for autotrophic biomass had values of YA = 2.3465 mg O2 mg N−1, μm, A = 0.7169 h−1, and KNH = 2.0748 mg N L−1.
AB - The moving bed biofilm reactor-membrane bioreactor (MBBR-MBR) is a novel solution to conventional activated sludge processes and membrane bioreactors. In this study, a pure MBBR-MBR was studied. The pure MBBR-MBR mainly had attached biomass. The bioreactor operated with a hydraulic retention time (HRT) of 9.5 h. The kinetic parameters for heterotrophic and autotrophic biomasses, mainly nitrite-oxidizing bacteria (NOB), were evaluated. The analysis of the bacterial community structure of the ammonium-oxidizing bacteria (AOB), NOB, and denitrifying bacteria (DeNB) from the pure MBBR-MBR was carried out by means of pyrosequencing to detect and quantify the contribution of the nitrifying and denitrifying bacteria in the total bacterial community. The relative abundance of AOB, NOB, and DeNB were 5, 1, and 3 %, respectively, in the mixed liquor suspended solids (MLSS), and these percentages were 18, 5, and 2 %, respectively, in the biofilm density (BD) attached to carriers. The pure MBBR-MBR had a high efficiency of total nitrogen (TN) removal of 71.81 ± 16.04 %, which could reside in the different bacterial assemblages in the fixed biofilm on the carriers. In this regard, the kinetic parameters for autotrophic biomass had values of YA = 2.3465 mg O2 mg N−1, μm, A = 0.7169 h−1, and KNH = 2.0748 mg N L−1.
KW - Autotrophic kinetics
KW - Biofilm
KW - Moving bed biofilm reactor
KW - Pyrosequencing
KW - Two-step nitrification
KW - Wastewater treatment
UR - http://www.scopus.com/inward/record.url?scp=84947022639&partnerID=8YFLogxK
U2 - 10.1007/s00253-015-6894-5
DO - 10.1007/s00253-015-6894-5
M3 - Article
C2 - 26264139
AN - SCOPUS:84947022639
VL - 99
SP - 10333
EP - 10343
JO - Applied Microbiology & Biotechnology
JF - Applied Microbiology & Biotechnology
SN - 0175-7598
IS - 23
ER -