Trapping of 27 bp-8 kbp DNA and immobilization of thiol-modified DNA using dielectrophoresis

Sampo Tuukkanen*, Anton Kuzyk, J. Jussi Toppari, Hannu Hakkinen, Vesa P. Hytönen, Einari Niskanen, Marcus Rinkio, Päivi Törmä

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Dielectrophoretic trapping of six different DNA fragments, sizes varying from 27 to 8416 bp, has been studied using confocal microscopy. The effect of the DNA length and the size of the constriction between nanoscale fingertip electrodes on the trapping efficiency have been investigated. Using finite element method simulations in conjunction with the analysis of the experimental data, the polarizabilities of the different size DNA fragments have been calculated for different frequencies. Also the immobilization of trapped hexanethiol- and DTPA-modified 140 nm long DNA to the end of gold nanoelectrodes was experimentally quantified and the observations were supported by density functional theory calculations.

Original languageEnglish
Article number295204
Pages (from-to)1-10
Number of pages10
JournalNanotechnology
Volume18
Issue number29
DOIs
Publication statusPublished - 25 Jul 2007
MoE publication typeA1 Journal article-refereed

Keywords

  • DOUBLE-STRANDED DNA
  • FLUID INTEGRATED-CIRCUIT
  • ELECTRIC-FIELDS
  • SUBMICRON BIOPARTICLES
  • DIELECTRIC-RELAXATION
  • CARBON NANOTUBES
  • MANIPULATION
  • CELLS
  • MICROELECTRODES
  • NANOPARTICLES

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