Transformation of Penicillin Resistance Plasmid from Penicillin Resistant soil Bacteria to E.coli DH5α

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Abstract: Transformation, an active uptake of free DNA by bacterial cells and the heritable incorporation of its genetic information is one of the most adventurous mechanism that can be manipulated with the technology to produce genetically modified and manipulated organisms that can fulfil the needs of the user.. Only more recently has it been considered that transformation may be a powerful mechanism of horizontal gene transfer in natural bacterial populations. With the advent of the technologies like RFLP, Amplification, Electrophoresis the act of addition and deletion of the genes into the native genome of the organisms has been taking up a major hand. The current work is an attempt to add the important antibiotic resistant R plasmid having penicillin resistance gene from the natural host to the E. coli cells which lack the property of Penicillin resistance. Once the transformation is performed the cells are cultured and tested for their antibiotic resistant property. If the cells are successfully transformed they too exhibit the resistant action against the antibiotic penicillin
Original languageEnglish
Pages (from-to)491- 493
Number of pages3
JournalHelix
Volume4
Issue number1
Publication statusPublished - 1 Jan 2014
MoE publication typeA1 Journal article-refereed

Keywords

  • transformation
  • Plasmid
  • penicillin gene
  • VECTOR

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