Transcript analysis of genes encoding a family 61 endoglucanase and a putative membrane-anchored family 9 glycosyl hydrolase from Phanerochaete chrysosporium

A Vanden Wymelenberg, S Denman, D Dietrich, J Bassett, XC Yu, R Atalla, P Predki, U Rudsander, TT Teeri, D Cullen*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Phanerochaete chrysosporium cellulase genes were cloned and characterized. The cel61A product was structurally similar to fungal endoglucanases of glycoside hydrolase family 61, whereas the cel9A product revealed similarities to Thermobifida fusca Cel9A (E4), an enzyme with both endo- and exocellulase characteristics. The fungal Cel9A is apparently a membrane-bound protein, which is very unusual for microbial cellulases. Transcript levels of both genes were substantially higher in cellulose-grown cultures than in glucose-grown cultures. These results show that P. chrysosporium possesses a wide array of conventional and unconventional cellulase genes.

Original languageEnglish
Pages (from-to)5765-5768
Number of pages4
JournalApplied and Environmental Microbiology
Volume68
Issue number11
DOIs
Publication statusPublished - Nov 2002
MoE publication typeA1 Journal article-refereed

Keywords

  • I-LIKE GENES
  • TRICHODERMA-REESEI
  • SPOROTRICHUM-PULVERULENTUM
  • DIFFERENTIAL EXPRESSION
  • ENZYME-SYSTEM
  • CELLULOSE
  • CELLOBIOHYDROLASE
  • PURIFICATION
  • ORGANIZATION
  • CLONING

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