Abstract
Phanerochaete chrysosporium cellulase genes were cloned and characterized. The cel61A product was structurally similar to fungal endoglucanases of glycoside hydrolase family 61, whereas the cel9A product revealed similarities to Thermobifida fusca Cel9A (E4), an enzyme with both endo- and exocellulase characteristics. The fungal Cel9A is apparently a membrane-bound protein, which is very unusual for microbial cellulases. Transcript levels of both genes were substantially higher in cellulose-grown cultures than in glucose-grown cultures. These results show that P. chrysosporium possesses a wide array of conventional and unconventional cellulase genes.
Original language | English |
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Pages (from-to) | 5765-5768 |
Number of pages | 4 |
Journal | Applied and Environmental Microbiology |
Volume | 68 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2002 |
MoE publication type | A1 Journal article-refereed |
Keywords
- I-LIKE GENES
- TRICHODERMA-REESEI
- SPOROTRICHUM-PULVERULENTUM
- DIFFERENTIAL EXPRESSION
- ENZYME-SYSTEM
- CELLULOSE
- CELLOBIOHYDROLASE
- PURIFICATION
- ORGANIZATION
- CLONING