Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface

Research output: Contribution to journalArticle

Researchers

  • Kiyohiko Igarashi
  • Takayuki Uchihashi
  • Anu Koivula
  • Masahisa Wada
  • Satoshi Kimura
  • Tetsuaki Okamoto
  • Merja Penttila
  • Toshio Ando
  • Masahiro Samejima

Research units

  • University of Tokyo
  • Japan Sci & Technol Agcy, Japan Science & Technology Agency (JST), Chiyoda Ku
  • VTT Technical Research Centre of Finland
  • Kyung Hee Univ, Kyung Hee University, Coll Life Sci, Dept Plant & Environm New Resources
  • Kanazawa University

Abstract

A deeper mechanistic understanding of the saccharification of cellulosic biomass could enhance the efficiency of biofuels development. We report here the real-time visualization of crystalline cellulose degradation by individual cellulase enzymes through use of an advanced version of high-speed atomic force microscopy. Trichoderma reesei cellobiohydrolase I (TrCel7A) molecules were observed to slide unidirectionally along the crystalline cellulose surface but at one point exhibited collective halting analogous to a traffic jam. Changing the crystalline polymorphic form of cellulose by means of an ammonia treatment increased the apparent number of accessible lanes on the crystalline surface and consequently the number of moving cellulase molecules. Treatment of this bulky crystalline cellulose simultaneously or separately with T. reesei cellobiohydrolase II (TrCel6A) resulted in a remarkable increase in the proportion of mobile enzyme molecules on the surface. Cellulose was completely degraded by the synergistic action between the two enzymes.

Details

Original languageEnglish
Pages (from-to)1279-1282
Number of pages4
JournalScience
Volume333
Issue number6047
Publication statusPublished - 2 Sep 2011
MoE publication typeA1 Journal article-refereed

    Research areas

  • NEUTRON FIBER DIFFRACTION, SYNCHROTRON X-RAY, TRICHODERMA-REESEI, CRYSTALLINE CELLULOSE, CELLOBIOHYDROLASE-I, ENZYMATIC-HYDROLYSIS, DEGRADATION, MICROCRYSTALS, MECHANISM, MODEL

ID: 9227932