Toxic indole alkaloids avrainvillamide and stephacidin B produced by a biocide tolerant indoor mold Aspergillus westerdijkiae

Raimo Mikkola*, Maria A. Andersson, Maria Hautaniemi, Mirja S. Salkinoja-Salonen

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

Toxic Aspergillus westerdijkiae were present in house dust and indoor air fall-out from a residence and a kindergarten where the occupants suffered from building related ill health. The A. westerdijkiae isolates produced indole alkaloids avrainvillamide (445 Da) and its dimer stephacidin B (890 Da). It grew and sporulated in presence of high concentrations of boron or polyguanidine (PHMB, PHMG) based antimicrobial biocides used to remediate mold infested buildings. The boar sperm cells were used as sensor cells to purify toxins from HPLC fractions of the fungal biomass. Submicromolar concentrations (EC50 0.3-0.4 mu M) blocked boar spermatozoan motility and killed porcine kidney tubular epithelial cells (PK-15). Plate grown hyphal mass of the A. westerdijkiae isolates contained 300-750 ng of avrainvillamide and 30-300 ng of stephacidin B per mg (wet weight). The toxins induced rapid (30 min) loss of boar sperm motility, followed (24 h) by loss of mitochondrial membrane potential (Delta Psi m). Apoptotic cell death was observed in PK-15 cell monolayers, prior to cessation of glucose uptake or loss of Delta Psi m. Avrainvillamide and stephacidin B were 100-fold more potent towards the porcine cells than the mycotoxins stephacidin A, ochratoxin A, sterigmatocystin and citrinin. The high toxicity of stephacidin B indicates a role of nitrone group in the mechanism of toxicity. Avrainvillamide and stephacidin B represent a new class of toxins with possible a threat to human health in buildings. Furthermore, the use of biocides highly enhanced the growth of toxigenic A. westerdijkiae. (C) 2015 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)58-67
Number of pages10
JournalTOXICON
Volume99
DOIs
Publication statusPublished - 1 Jun 2015
MoE publication typeA1 Journal article-refereed

Keywords

  • Indoor mold
  • Biocide tolerance
  • Aspergillus
  • Indole alkaloid
  • Mitochondriotoxin
  • Nitrone
  • MARINE-DERIVED FUNGUS
  • BUILDING-MATERIALS
  • MAMMALIAN-CELLS
  • OCHRATOXIN-A
  • FUMIGATUS
  • MYCOTOXINS
  • SPERM
  • MITOCHONDRIA
  • CJ-17,665
  • APOPTOSIS

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