Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity

Soumyananda Chakraborti*, Antti Korpi, Mantu Kumar, Piotr Stȩpień, Mauri A. Kostiainen, Jonathan G. Heddle

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

27 Citations (Scopus)
59 Downloads (Pure)

Abstract

Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.

Original languageEnglish
Pages (from-to)3918-3924
Number of pages7
JournalNano Letters
Volume19
Issue number6
DOIs
Publication statusPublished - Jun 2019
MoE publication typeA1 Journal article-refereed

Keywords

  • 3D enzyme array
  • chaperone activity
  • enzyme encapsulation
  • Ferritin
  • ferritin superlattice
  • NANOREACTOR
  • COCRYSTALS
  • ARCHAEOGLOBUS-FULGIDUS FERRITIN
  • ENCAPSULATION
  • APOFERRITIN
  • RESIDUES

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