Three complementary techniques for the clarification of temperature effect on low-density lipoprotein-chondroitin-6-sulfate interaction

Geraldine Cilpa-Karhu*, Katriina Lipponen, Jörgen Samuelsson, Katariina Öörni, Torgny Fornstedt, Marja-Liisa Riekkola

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

A rigorous processing of adsorption data from quartz crystal microbalance technology was successfully combined with the data obtained by partial filling affinity capillary electrophoresis and molecular dynamics for the clarification of the temperature effect on the interaction of a major glycosaminoglycan chain chondroitin-6-sulfate (C6S) of proteoglycans with low-density lipoprotein (LDL) and with a peptide fragment of apolipoprotein B-100 (residues 3359-3377 of LDL, PPBS). Two experimental techniques and computational atomistic methods demonstrated a nonlinear pattern of the affinity of C6S at temperatures above 38.0 C to both LDL and PPBS. The temperature affects the interaction of C6S with LDL and PPBS by influencing the structural behavior of glycosaminoglycan C6S and/or that of LDL.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalANALYTICAL BIOCHEMISTRY
Volume443
Issue number2
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Keywords

  • Adsorption energy distribution
  • Glycosaminoglycan
  • Low-density lipoprotein
  • Molecular dynamics simulations
  • Partial filling affinity capillary electrophoresis
  • Quartz crystal microbalance

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