Thiol-ene-based, replicated, high-aspect ratio micropillar arrays as immobilized chymotrypsin reactors

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientificpeer-review

Researchers

  • Sari Tähkä
  • Riccardo Provenzani
  • Gustav Boije Af Gennäs
  • Jari Yli-Kauhaluoma
  • Ville Jokinen
  • Tiina Sikanen

Research units

  • University of Helsinki

Abstract

We present a new non-cleanroom-based replication approach for implementation of high aspect ratio (h=200 μm, d=50 μm) micropillar arrays out of thiol-enes followed by biofunctionalization with chymotrypsin. Our approach exploits gold nanoparticles (GNPs) to bind the enzymes on the thiol-ene surfaces via gold-thiol chemistry.

Details

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publication statusPublished - 2016
MoE publication typeA4 Article in a conference publication
EventInternational Conference on Miniaturized Systems for Chemistry and Life Sciences - Dublin, Ireland
Duration: 9 Oct 201613 Oct 2016
Conference number: 20

Conference

ConferenceInternational Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated titleMicroTAS 2016
CountryIreland
CityDublin
Period09/10/201613/10/2016

    Research areas

  • Gold nanoparticles, Immobilized enzyme microreactors, Replica molding, Thiol-enes

ID: 16821740