Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis

Katriina Lipponen, Sari Tähkä, T. Sikanen, V. Jokinen, A. Tatikonda, S. Franssila, R. Kostiainen, T. Kotiaho*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

4 Citations (Scopus)

Abstract

A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10-20 mol) for a peptide standard.

Original languageEnglish
Pages (from-to)2552-2557
Number of pages6
JournalAnalyst
Volume142
Issue number14
DOIs
Publication statusPublished - 21 Jul 2017
MoE publication typeA1 Journal article-refereed

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