Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis

Katriina Lipponen; Sari Tähkä; T. Sikanen; V. Jokinen; A. Tatikonda; S. Franssila; R. Kostiainen; T. Kotiaho

doi:10.1039/c7an00544j

Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis

Katriina Lipponen, Sari Tähkä, T. Sikanen, V. Jokinen, A. Tatikonda, S. Franssila, R. Kostiainen, T. Kotiaho^*

^*Corresponding author for this work

University of Helsinki

Research output: Contribution to journal › Article › Scientific › peer-review

5 Citations (Scopus)

Abstract

A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10^-20 mol) for a peptide standard.

Original language	English
Pages (from-to)	2552-2557
Number of pages	6
Journal	Analyst
Volume	142
Issue number	14
DOIs	https://doi.org/10.1039/c7an00544j
Publication status	Published - 21 Jul 2017
MoE publication type	A1 Journal article-refereed

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10.1039/c7an00544j

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title = "Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis",

abstract = "A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10-20 mol) for a peptide standard.",

author = "Katriina Lipponen and Sari T{\"a}hk{\"a} and T. Sikanen and V. Jokinen and A. Tatikonda and S. Franssila and R. Kostiainen and T. Kotiaho",

year = "2017",

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doi = "10.1039/c7an00544j",

language = "English",

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T1 - Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis

AU - Lipponen, Katriina

AU - Tähkä, Sari

AU - Sikanen, T.

AU - Jokinen, V.

AU - Tatikonda, A.

AU - Franssila, S.

AU - Kostiainen, R.

AU - Kotiaho, T.

PY - 2017/7/21

Y1 - 2017/7/21

N2 - A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10-20 mol) for a peptide standard.

AB - A micropillar array electrospray ionization (μPESI) platform fabricated from thiol-enes with 56 individual polyethylene glycol coated μPESI chips for bioanalytical mass spectrometry is introduced. Bioanalysis capability is shown by measurement of a protein, a protein digest and a cell lysate sample. The thiol-ene polyethylene glycol (PEG) coated μPESI chip allows the use of a wide range of aqueous-organic solvent compositions and provides a detection limit at 60 zeptomole level (6 × 10-20 mol) for a peptide standard.

UR - http://www.scopus.com/inward/record.url?scp=85022332486&partnerID=8YFLogxK

U2 - 10.1039/c7an00544j

DO - 10.1039/c7an00544j

M3 - Article

AN - SCOPUS:85022332486

SN - 0003-2654

VL - 142

SP - 2552

EP - 2557

JO - Analyst

JF - Analyst

IS - 14

ER -

Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis

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Thiol-ene micropillar array electrospray ionization platform for zeptomole level bioanalysis

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