Thiol-ene-based, replicated, high-aspect ratio micropillar arrays as immobilized chymotrypsin reactors

Sari Tähkä; Riccardo Provenzani; Gustav Boije Af Gennäs; Jari Yli-Kauhaluoma; Ville Jokinen; Tiina Sikanen

Thiol-ene-based, replicated, high-aspect ratio micropillar arrays as immobilized chymotrypsin reactors

Sari Tähkä^*, Riccardo Provenzani, Gustav Boije Af Gennäs, Jari Yli-Kauhaluoma, Ville Jokinen, Tiina Sikanen

^*Corresponding author for this work

University of Helsinki

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

Abstract

We present a new non-cleanroom-based replication approach for implementation of high aspect ratio (h=200 μm, d=50 μm) micropillar arrays out of thiol-enes followed by biofunctionalization with chymotrypsin. Our approach exploits gold nanoparticles (GNPs) to bind the enzymes on the thiol-ene surfaces via gold-thiol chemistry.

Original language	English
Title of host publication	20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Publisher	Chemical and Biological Microsystems Society (CBMS)
Pages	1491-1492
Number of pages	2
ISBN (Electronic)	9780979806490
Publication status	Published - 2016
MoE publication type	A4 Conference publication
Event	International Conference on Miniaturized Systems for Chemistry and Life Sciences - Dublin, Ireland Duration: 9 Oct 2016 → 13 Oct 2016 Conference number: 20

Conference

Conference	International Conference on Miniaturized Systems for Chemistry and Life Sciences
Abbreviated title	MicroTAS 2016
Country/Territory	Ireland
City	Dublin
Period	09/10/2016 → 13/10/2016

Keywords

Gold nanoparticles
Immobilized enzyme microreactors
Replica molding
Thiol-enes

Cite this

Tähkä, S., Provenzani, R., Af Gennäs, G. B., Yli-Kauhaluoma, J., Jokinen, V., & Sikanen, T. (2016). Thiol-ene-based, replicated, high-aspect ratio micropillar arrays as immobilized chymotrypsin reactors. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 1491-1492). Chemical and Biological Microsystems Society (CBMS).

@inproceedings{23b46053e8a74ef397ad65ad83e41341,

title = "Thiol-ene-based, replicated, high-aspect ratio micropillar arrays as immobilized chymotrypsin reactors",

abstract = "We present a new non-cleanroom-based replication approach for implementation of high aspect ratio (h=200 μm, d=50 μm) micropillar arrays out of thiol-enes followed by biofunctionalization with chymotrypsin. Our approach exploits gold nanoparticles (GNPs) to bind the enzymes on the thiol-ene surfaces via gold-thiol chemistry.",

keywords = "Gold nanoparticles, Immobilized enzyme microreactors, Replica molding, Thiol-enes",

author = "Sari T{\"a}hk{\"a} and Riccardo Provenzani and {Af Genn{\"a}s}, {Gustav Boije} and Jari Yli-Kauhaluoma and Ville Jokinen and Tiina Sikanen",

year = "2016",

language = "English",

pages = "1491--1492",

booktitle = "20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016",

publisher = "Chemical and Biological Microsystems Society (CBMS)",

address = "United States",

note = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 ; Conference date: 09-10-2016 Through 13-10-2016",

}

Tähkä, S, Provenzani, R, Af Gennäs, GB, Yli-Kauhaluoma, J, Jokinen, V & Sikanen, T 2016, Thiol-ene-based, replicated, high-aspect ratio micropillar arrays as immobilized chymotrypsin reactors. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society (CBMS), pp. 1491-1492, International Conference on Miniaturized Systems for Chemistry and Life Sciences, Dublin, Ireland, 09/10/2016.

Thiol-ene-based, replicated, high-aspect ratio micropillar arrays as immobilized chymotrypsin reactors. / Tähkä, Sari; Provenzani, Riccardo; Af Gennäs, Gustav Boije et al.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society (CBMS), 2016. p. 1491-1492.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

TY - GEN

T1 - Thiol-ene-based, replicated, high-aspect ratio micropillar arrays as immobilized chymotrypsin reactors

AU - Tähkä, Sari

AU - Provenzani, Riccardo

AU - Af Gennäs, Gustav Boije

AU - Yli-Kauhaluoma, Jari

AU - Jokinen, Ville

AU - Sikanen, Tiina

N1 - Conference code: 20

PY - 2016

Y1 - 2016

N2 - We present a new non-cleanroom-based replication approach for implementation of high aspect ratio (h=200 μm, d=50 μm) micropillar arrays out of thiol-enes followed by biofunctionalization with chymotrypsin. Our approach exploits gold nanoparticles (GNPs) to bind the enzymes on the thiol-ene surfaces via gold-thiol chemistry.

AB - We present a new non-cleanroom-based replication approach for implementation of high aspect ratio (h=200 μm, d=50 μm) micropillar arrays out of thiol-enes followed by biofunctionalization with chymotrypsin. Our approach exploits gold nanoparticles (GNPs) to bind the enzymes on the thiol-ene surfaces via gold-thiol chemistry.

KW - Gold nanoparticles

KW - Immobilized enzyme microreactors

KW - Replica molding

KW - Thiol-enes

UR - http://www.scopus.com/inward/record.url?scp=85014197554&partnerID=8YFLogxK

M3 - Conference article in proceedings

AN - SCOPUS:85014197554

SP - 1491

EP - 1492

BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

PB - Chemical and Biological Microsystems Society (CBMS)

T2 - International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 9 October 2016 through 13 October 2016

ER -

Thiol-ene-based, replicated, high-aspect ratio micropillar arrays as immobilized chymotrypsin reactors

Abstract

Conference

Keywords

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