The 'true' L-xylulose reductase of filamentous fungi identified in Aspergillus niger

Dominik Mojzita, Kiira Vuoristo, Outi M. Koivistoinen, Merja Penttila, Peter Richard*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

L-Xylulose reductase is part of the eukaryotic pathway for L-arabinose catabolism. A previously identified L-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on L-arabinose and the deletion strain showed no reduced L-xylulose reductase activity but instead lost the D-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' L-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on L-arabinose and the deletion results in a strain lacking the NADPH-specific L-xylulose reductase activity and having reduced growth on L-arabinose. The purified enzyme had a K(m) for L-xylulose of 25 mM and a nu(max) of 650 U/mg. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Original languageEnglish
Pages (from-to)3540-3544
Number of pages5
JournalFEBS Letters
Volume584
Issue number16
DOIs
Publication statusPublished - 20 Aug 2010
MoE publication typeA1 Journal article-refereed

Keywords

  • EC 1.1.1.10
  • L-Arabinose metabolism
  • L-Xylulose
  • Pentose
  • Aspergillus niger
  • L-ARABINOSE METABOLISM
  • HYPOCREA-JECORINA
  • CATABOLIC PATHWAY
  • GENE
  • DEHYDROGENASE
  • EXPRESSION
  • CLONING
  • PENTOSE
  • YEAST

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