The influence of surface chemical composition on the adsorption of xyloglucan to chemical and mechanical pulps

Q Zhou, MJ Baumann, H Brumer*, TT Teeri

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Adsorption kinetics of xyloglucan (XG) onto bleached chemical and mechanical wood pulps were studied to better understand the application of this plant polysaccharide to the modification of key industrial cellulosic materials. Bleached pulps prepared by the kraft process adsorbed significantly larger amounts of xyloglucan than did thermomechanical pulps (TMP) and chemi-thermomechanical pulps (CTMP). The ability of pulps to adsorb xyloglucan was intimately related to the surface amounts Of Cellulose, lignin and extractives, as determined by electron spectroscopy for chemical analysis (ESCA). The distribution of absorbed XG on bleached Pulp fibers was examined with an XG-fluorescein conjugate (XG-FITC), which was prepared by xyloglucan endotransglycosylase (XET, EC 2.4.1.207)-mediated incorporation of fluoresce-inlabeled xyloglucan oligosaccharides (XGO-FITC) into XG. Fluorescence microscopy revealed that XG-FITC preferentially adsorbed on fiber surfaces, and was adsorbed in higher amounts to chemical pulps than mechanical pulps, as observed for the unmodified parent polysaccharide. Addition of hybrid aspen XET (PttXET16A) and XGO-FITC to bleached and unbleached mechanical Pulps indicated that the amount of enzyme-accessible xyloglucan presented on TMP and CTMP surfaces is low. (C) 2005 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)449-458
Number of pages10
JournalCarbohydrate Polymers
Volume63
Issue number4
DOIs
Publication statusPublished - 18 Mar 2006
MoE publication typeA1 Journal article-refereed

Keywords

  • xyloglucan
  • xyloglucan endotransglycosylase (XET)
  • chemical and mechanical pulp fiber
  • surface chemical composition
  • fluorescence microscopy
  • cellulose
  • wood pulp
  • fiber modification
  • surface chemistry
  • green chemistry
  • PLANT-CELL WALLS
  • CELLULOSE FIBER SURFACES
  • SIDE-CHAINS
  • KRAFT PULPS
  • ENDOTRANSGLYCOSYLASE
  • OLIGOSACCHARIDES
  • CHEMISTRY
  • TAMARIND
  • BINDING
  • ESCA

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