The genome sequence of black cottonwood (Populus trichocarpa) reveals 18 conserved cellulose synthase (CesA) genes

Research output: Contribution to journalArticle

Researchers

  • S Djerbi
  • M Lindskog
  • L Arvestad
  • F Sterky
  • TT Teeri

Research units

  • KTH Royal Institute of Technology

Abstract

The genome sequence of Populus trichocarpa was screened for genes encoding cellulose synthases by using full-length cDNA sequences and ESTs previously identified in the tissue specific cDNA libraries of other poplars. The data obtained revealed 18 distinct CesA gene sequences in P. trichocarpa. The identified genes were grouped in seven gene pairs, one group of three sequences and one single gene. Evidence from gene expression studies of hybrid aspen suggests that both copies of at least one pair, CesA3-1 and CesA3-2, are actively transcribed. No sequences corresponding to the gene pair, CesA6-1 and CesA6-2, were found in Arabidopsis or hybrid aspen, while one homologous gene has been identified in the rice genome and an active transcript in Populus tremuloides. A phylogenetic analysis suggests that the CesA genes previously associated with secondary cell wall synthesis originate from a single ancestor gene and group in three distinct subgroups. The newly identified copies of CesA genes in P. trichocarpa give rise to a number of new questions concerning the mechanism of cellulose synthesis in trees.

Details

Original languageEnglish
Pages (from-to)739-746
Number of pages8
JournalPLANTA
Volume221
Issue number5
Publication statusPublished - Jul 2005
MoE publication typeA1 Journal article-refereed

    Research areas

  • Populus, paleopolyploids, cellulose synthase, gene duplication, paralogues, FUNCTIONAL GENOMICS, ARABIDOPSIS, ALIGNMENT, FAMILY, BIOSYNTHESIS, RESISTANCE, EVOLUTION, ISOXABEN, SEARCH, ASPEN

ID: 2056723