The diverse role of Pdr12 in resistance to weak organic acids

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The diverse role of Pdr12 in resistance to weak organic acids. / Nygård, Yvonne; Mojzita, Dominik; Toivari, Mervi; Penttilä, Merja; Wiebe, Marilyn G.; Ruohonen, Laura.

In: Yeast, Vol. 31, No. 6, 06.2014, p. 219-232.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Nygård, Y, Mojzita, D, Toivari, M, Penttilä, M, Wiebe, MG & Ruohonen, L 2014, 'The diverse role of Pdr12 in resistance to weak organic acids', Yeast, vol. 31, no. 6, pp. 219-232. https://doi.org/10.1002/yea.3011

APA

Nygård, Y., Mojzita, D., Toivari, M., Penttilä, M., Wiebe, M. G., & Ruohonen, L. (2014). The diverse role of Pdr12 in resistance to weak organic acids. Yeast, 31(6), 219-232. https://doi.org/10.1002/yea.3011

Vancouver

Nygård Y, Mojzita D, Toivari M, Penttilä M, Wiebe MG, Ruohonen L. The diverse role of Pdr12 in resistance to weak organic acids. Yeast. 2014 Jun;31(6):219-232. https://doi.org/10.1002/yea.3011

Author

Nygård, Yvonne ; Mojzita, Dominik ; Toivari, Mervi ; Penttilä, Merja ; Wiebe, Marilyn G. ; Ruohonen, Laura. / The diverse role of Pdr12 in resistance to weak organic acids. In: Yeast. 2014 ; Vol. 31, No. 6. pp. 219-232.

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@article{fe4819bca7594eb1ac9aa1ff384c683f,
title = "The diverse role of Pdr12 in resistance to weak organic acids",
abstract = "Resistance to weak organic acids is important relative to both weak organic acid preservatives and the development of inhibitor tolerant yeast as industrial production organisms. The ABC transporter Pdr12 is important for resistance to sorbic and propionic acid, but its role in tolerance to other weak organic acids with industrial relevance is not well established. In this study, yeast strains with altered expression of PDR12 and/or CMK1, a protein kinase associated with post-transcriptional negative regulation of Pdr12, were exposed to seven weak organic acids: acetic, formic, glycolic, lactic, propionic, sorbic and levulinic acid. These are widely used as preservatives, present in lignocellulosic hydrolysates or attractive as chemical precursors. Overexpression of PDR12 increased tolerance to acids with longer chain length, such as sorbic, propionic and levulinic acid, whereas deletion of PDR12 increased tolerance to the shorter acetic and formic acid. The viability of all strains decreased dramatically in acetic or propionic acid, but the pdr12 strains recovered more rapidly than other strains in acetic acid. Furthermore, our results indicated that Cmk1 plays a role in weak organic acid tolerance, beyond its role in regulation of Pdr12, since deletion of both Cmk1 and Pdr12 resulted in different responses to exposure to acids than were explained by deletion of Pdr12 alone. Copyright (c) 2014 John Wiley & Sons, Ltd.",
keywords = "Saccharomyces cerevisiae, weak organic acid tolerance, Pdr12, plasma membrane transporters, Cmk1, BINDING CASSETTE TRANSPORTER, MEMBRANE H+-ATPASE, SACCHAROMYCES-CEREVISIAE, PROTEIN-KINASE, ABC TRANSPORTER, SORBIC ACID, FOOD PRESERVATIVES, ADAPTIVE RESPONSE, GROWTH-INHIBITION, D-XYLONATE",
author = "Yvonne Nyg{\aa}rd and Dominik Mojzita and Mervi Toivari and Merja Penttil{\"a} and Wiebe, {Marilyn G.} and Laura Ruohonen",
year = "2014",
month = "6",
doi = "10.1002/yea.3011",
language = "English",
volume = "31",
pages = "219--232",
journal = "Yeast",
issn = "0749-503X",
number = "6",

}

RIS - Download

TY - JOUR

T1 - The diverse role of Pdr12 in resistance to weak organic acids

AU - Nygård, Yvonne

AU - Mojzita, Dominik

AU - Toivari, Mervi

AU - Penttilä, Merja

AU - Wiebe, Marilyn G.

AU - Ruohonen, Laura

PY - 2014/6

Y1 - 2014/6

N2 - Resistance to weak organic acids is important relative to both weak organic acid preservatives and the development of inhibitor tolerant yeast as industrial production organisms. The ABC transporter Pdr12 is important for resistance to sorbic and propionic acid, but its role in tolerance to other weak organic acids with industrial relevance is not well established. In this study, yeast strains with altered expression of PDR12 and/or CMK1, a protein kinase associated with post-transcriptional negative regulation of Pdr12, were exposed to seven weak organic acids: acetic, formic, glycolic, lactic, propionic, sorbic and levulinic acid. These are widely used as preservatives, present in lignocellulosic hydrolysates or attractive as chemical precursors. Overexpression of PDR12 increased tolerance to acids with longer chain length, such as sorbic, propionic and levulinic acid, whereas deletion of PDR12 increased tolerance to the shorter acetic and formic acid. The viability of all strains decreased dramatically in acetic or propionic acid, but the pdr12 strains recovered more rapidly than other strains in acetic acid. Furthermore, our results indicated that Cmk1 plays a role in weak organic acid tolerance, beyond its role in regulation of Pdr12, since deletion of both Cmk1 and Pdr12 resulted in different responses to exposure to acids than were explained by deletion of Pdr12 alone. Copyright (c) 2014 John Wiley & Sons, Ltd.

AB - Resistance to weak organic acids is important relative to both weak organic acid preservatives and the development of inhibitor tolerant yeast as industrial production organisms. The ABC transporter Pdr12 is important for resistance to sorbic and propionic acid, but its role in tolerance to other weak organic acids with industrial relevance is not well established. In this study, yeast strains with altered expression of PDR12 and/or CMK1, a protein kinase associated with post-transcriptional negative regulation of Pdr12, were exposed to seven weak organic acids: acetic, formic, glycolic, lactic, propionic, sorbic and levulinic acid. These are widely used as preservatives, present in lignocellulosic hydrolysates or attractive as chemical precursors. Overexpression of PDR12 increased tolerance to acids with longer chain length, such as sorbic, propionic and levulinic acid, whereas deletion of PDR12 increased tolerance to the shorter acetic and formic acid. The viability of all strains decreased dramatically in acetic or propionic acid, but the pdr12 strains recovered more rapidly than other strains in acetic acid. Furthermore, our results indicated that Cmk1 plays a role in weak organic acid tolerance, beyond its role in regulation of Pdr12, since deletion of both Cmk1 and Pdr12 resulted in different responses to exposure to acids than were explained by deletion of Pdr12 alone. Copyright (c) 2014 John Wiley & Sons, Ltd.

KW - Saccharomyces cerevisiae

KW - weak organic acid tolerance

KW - Pdr12

KW - plasma membrane transporters

KW - Cmk1

KW - BINDING CASSETTE TRANSPORTER

KW - MEMBRANE H+-ATPASE

KW - SACCHAROMYCES-CEREVISIAE

KW - PROTEIN-KINASE

KW - ABC TRANSPORTER

KW - SORBIC ACID

KW - FOOD PRESERVATIVES

KW - ADAPTIVE RESPONSE

KW - GROWTH-INHIBITION

KW - D-XYLONATE

U2 - 10.1002/yea.3011

DO - 10.1002/yea.3011

M3 - Article

VL - 31

SP - 219

EP - 232

JO - Yeast

JF - Yeast

SN - 0749-503X

IS - 6

ER -

ID: 9226466