The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes

Olanrewaju Raji; Jenny Arnling Bååth; Thu V. Vuong; Johan Larsbrink; Lisbeth Olsson; Emma R. Master

doi:10.1002/1873-3468.14019

The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes

Olanrewaju Raji, Jenny Arnling Bååth, Thu V. Vuong, Johan Larsbrink, Lisbeth Olsson^*, Emma R. Master

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Glucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase (TtCE15A) and two α-glucuronidases (SdeAgu115A and AxyAgu115A). When supplementing α-glucuronidase with equimolar quantities of TtCE15A, total MeGlcpA released after 72 h by SdeAgu115A and AxyAgu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined TtCE15A and AxyAgu115A activities, ~ 34% of MeGlcpA in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α-glucuronidase concentrations by up to 70%, whereas reduction in soluble TtCE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.

Original language	English
Pages (from-to)	351-359
Number of pages	9
Journal	FEBS Letters
Volume	595
Issue number	3
Early online date	4 Dec 2020
DOIs	https://doi.org/10.1002/1873-3468.14019
Publication status	Published - Feb 2021
MoE publication type	A1 Journal article-refereed

Keywords

4-O-methyl d-glucuronic acid
carbohydrate-active enzymes
enzyme adsorption
glucuronoxylan
hemicellulases
lignin–carbohydrate complexes

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10.1002/1873-3468.14019

1873-3468.14019Final published version, 460 KBLicence: Unspecified

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title = "The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes",

abstract = "Glucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase (TtCE15A) and two α-glucuronidases (SdeAgu115A and AxyAgu115A). When supplementing α-glucuronidase with equimolar quantities of TtCE15A, total MeGlcpA released after 72 h by SdeAgu115A and AxyAgu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined TtCE15A and AxyAgu115A activities, ~ 34% of MeGlcpA in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α-glucuronidase concentrations by up to 70%, whereas reduction in soluble TtCE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.",

keywords = "4-O-methyl d-glucuronic acid, carbohydrate-active enzymes, enzyme adsorption, glucuronoxylan, hemicellulases, lignin–carbohydrate complexes",

author = "Olanrewaju Raji and {Arnling B{\aa}{\aa}th}, Jenny and Vuong, {Thu V.} and Johan Larsbrink and Lisbeth Olsson and Master, {Emma R.}",

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AU - Raji, Olanrewaju

AU - Arnling Bååth, Jenny

AU - Vuong, Thu V.

AU - Larsbrink, Johan

AU - Olsson, Lisbeth

AU - Master, Emma R.

N1 - | openaire: EC/H2020/648925/EU//BHIVE

PY - 2021/2

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N2 - Glucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase (TtCE15A) and two α-glucuronidases (SdeAgu115A and AxyAgu115A). When supplementing α-glucuronidase with equimolar quantities of TtCE15A, total MeGlcpA released after 72 h by SdeAgu115A and AxyAgu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined TtCE15A and AxyAgu115A activities, ~ 34% of MeGlcpA in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α-glucuronidase concentrations by up to 70%, whereas reduction in soluble TtCE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.

AB - Glucuronoxylans represent a significant fraction of woody biomass, and its decomposition is complicated by the presence of lignin–carbohydrate complexes (LCCs). Herein, LCCs from birchwood were used to investigate the potential coordinated action of a glucuronoyl esterase (TtCE15A) and two α-glucuronidases (SdeAgu115A and AxyAgu115A). When supplementing α-glucuronidase with equimolar quantities of TtCE15A, total MeGlcpA released after 72 h by SdeAgu115A and AxyAgu115A increased from 52% to 67%, and 61% to 95%, respectively. Based on the combined TtCE15A and AxyAgu115A activities, ~ 34% of MeGlcpA in the extracted birchwood glucuronoxylan was occupied as LCCs. Notably, insoluble LCC fractions reduced soluble α-glucuronidase concentrations by up to 70%, whereas reduction in soluble TtCE15A was less than 30%, indicating different tendencies to adsorb onto the LCC substrate.

KW - 4-O-methyl d-glucuronic acid

KW - carbohydrate-active enzymes

KW - enzyme adsorption

KW - glucuronoxylan

KW - hemicellulases

KW - lignin–carbohydrate complexes

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The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes

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The coordinated action of glucuronoyl esterase and alpha-glucuronidase promotes the disassembly of lignin-carbohydrate complexes

BHIVE: Bio-derived HIgh Value polymers through novel Enzyme function

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The coordinated action of glucuronoyl esterase and α-glucuronidase promotes the disassembly of lignin–carbohydrate complexes

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The coordinated action of glucuronoyl esterase and alpha-glucuronidase promotes the disassembly of lignin-carbohydrate complexes

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BHIVE: Bio-derived HIgh Value polymers through novel Enzyme function

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