TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells

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TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells. / Tsagaratou, Ageliki; González-Avalos, Edahí; Rautio, Sini; Scott-Browne, James P.; Togher, Susan; Pastor, William A.; Rothenberg, Ellen V.; Chavez, Lukas; Lähdesmäki, Harri; Rao, Anjana.

In: NATURE IMMUNOLOGY, Vol. 18, No. 1, 2017, p. 45-53.

Research output: Contribution to journalArticle

Harvard

Tsagaratou, A, González-Avalos, E, Rautio, S, Scott-Browne, JP, Togher, S, Pastor, WA, Rothenberg, EV, Chavez, L, Lähdesmäki, H & Rao, A 2017, 'TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells', NATURE IMMUNOLOGY, vol. 18, no. 1, pp. 45-53. https://doi.org/10.1038/ni.3630

APA

Tsagaratou, A., González-Avalos, E., Rautio, S., Scott-Browne, J. P., Togher, S., Pastor, W. A., ... Rao, A. (2017). TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells. NATURE IMMUNOLOGY, 18(1), 45-53. https://doi.org/10.1038/ni.3630

Vancouver

Tsagaratou A, González-Avalos E, Rautio S, Scott-Browne JP, Togher S, Pastor WA et al. TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells. NATURE IMMUNOLOGY. 2017;18(1):45-53. https://doi.org/10.1038/ni.3630

Author

Tsagaratou, Ageliki ; González-Avalos, Edahí ; Rautio, Sini ; Scott-Browne, James P. ; Togher, Susan ; Pastor, William A. ; Rothenberg, Ellen V. ; Chavez, Lukas ; Lähdesmäki, Harri ; Rao, Anjana. / TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells. In: NATURE IMMUNOLOGY. 2017 ; Vol. 18, No. 1. pp. 45-53.

Bibtex - Download

@article{be669c68cbef4bb9be799835a43f2333,
title = "TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells",
abstract = "TET proteins oxidize 5-methylcytosine in DNA to 5-hydroxymethylcytosine and other oxidation products. We found that simultaneous deletion of Tet2 and Tet3 in mouse CD4+CD8+ double-positive thymocytes resulted in dysregulated development and proliferation of invariant natural killer T cells (iNKT cells). Tet2-Tet3 double-knockout (DKO) iNKT cells displayed pronounced skewing toward the NKT17 lineage, with increased DNA methylation and impaired expression of genes encoding the key lineage-specifying factors T-bet and ThPOK. Transfer of purified Tet2-Tet3 DKO iNKT cells into immunocompetent recipient mice resulted in an uncontrolled expansion that was dependent on the nonclassical major histocompatibility complex (MHC) protein CD1d, which presents lipid antigens to iNKT cells. Our data indicate that TET proteins regulate iNKT cell fate by ensuring their proper development and maturation and by suppressing aberrant proliferation mediated by the T cell antigen receptor (TCR).",
author = "Ageliki Tsagaratou and Edah{\'i} Gonz{\'a}lez-Avalos and Sini Rautio and Scott-Browne, {James P.} and Susan Togher and Pastor, {William A.} and Rothenberg, {Ellen V.} and Lukas Chavez and Harri L{\"a}hdesm{\"a}ki and Anjana Rao",
year = "2017",
doi = "10.1038/ni.3630",
language = "English",
volume = "18",
pages = "45--53",
journal = "NATURE IMMUNOLOGY",
issn = "1529-2908",
publisher = "Nature Publishing Group",
number = "1",

}

RIS - Download

TY - JOUR

T1 - TET proteins regulate the lineage specification and TCR-mediated expansion of iNKT cells

AU - Tsagaratou, Ageliki

AU - González-Avalos, Edahí

AU - Rautio, Sini

AU - Scott-Browne, James P.

AU - Togher, Susan

AU - Pastor, William A.

AU - Rothenberg, Ellen V.

AU - Chavez, Lukas

AU - Lähdesmäki, Harri

AU - Rao, Anjana

PY - 2017

Y1 - 2017

N2 - TET proteins oxidize 5-methylcytosine in DNA to 5-hydroxymethylcytosine and other oxidation products. We found that simultaneous deletion of Tet2 and Tet3 in mouse CD4+CD8+ double-positive thymocytes resulted in dysregulated development and proliferation of invariant natural killer T cells (iNKT cells). Tet2-Tet3 double-knockout (DKO) iNKT cells displayed pronounced skewing toward the NKT17 lineage, with increased DNA methylation and impaired expression of genes encoding the key lineage-specifying factors T-bet and ThPOK. Transfer of purified Tet2-Tet3 DKO iNKT cells into immunocompetent recipient mice resulted in an uncontrolled expansion that was dependent on the nonclassical major histocompatibility complex (MHC) protein CD1d, which presents lipid antigens to iNKT cells. Our data indicate that TET proteins regulate iNKT cell fate by ensuring their proper development and maturation and by suppressing aberrant proliferation mediated by the T cell antigen receptor (TCR).

AB - TET proteins oxidize 5-methylcytosine in DNA to 5-hydroxymethylcytosine and other oxidation products. We found that simultaneous deletion of Tet2 and Tet3 in mouse CD4+CD8+ double-positive thymocytes resulted in dysregulated development and proliferation of invariant natural killer T cells (iNKT cells). Tet2-Tet3 double-knockout (DKO) iNKT cells displayed pronounced skewing toward the NKT17 lineage, with increased DNA methylation and impaired expression of genes encoding the key lineage-specifying factors T-bet and ThPOK. Transfer of purified Tet2-Tet3 DKO iNKT cells into immunocompetent recipient mice resulted in an uncontrolled expansion that was dependent on the nonclassical major histocompatibility complex (MHC) protein CD1d, which presents lipid antigens to iNKT cells. Our data indicate that TET proteins regulate iNKT cell fate by ensuring their proper development and maturation and by suppressing aberrant proliferation mediated by the T cell antigen receptor (TCR).

UR - http://www.scopus.com/inward/record.url?scp=84996836078&partnerID=8YFLogxK

U2 - 10.1038/ni.3630

DO - 10.1038/ni.3630

M3 - Article

VL - 18

SP - 45

EP - 53

JO - NATURE IMMUNOLOGY

JF - NATURE IMMUNOLOGY

SN - 1529-2908

IS - 1

ER -

ID: 9730061