Tension wood structure and morphology conducive for better enzymatic digestion

Research output: Contribution to journalArticleScientificpeer-review

Researchers

  • Daisuke Sawada

  • Udaya C. Kalluri
  • Hugh O'Neill
  • Volker Urban
  • Paul Langan
  • Brian Davison
  • Sai Venkatesh Pingali

Research units

  • Oak Ridge National Laboratory

Abstract

Background: Tension wood is a type of reaction wood in response to bending or leaning stem as a corrective growth process. Tension wood is formed by both natural and man-made processes. Most attractively, tension wood contains higher glucan content and undergoes higher enzymatic conversion to fermentable sugars. Here, we have employed structural techniques, small-angle neutron scattering (SANS) and wide-angle X-ray diffraction (WAXD) to elucidate structural and morphological aspects of tension wood conducive to higher sugar yields. Results: Small-angle neutron scattering data exhibited a tri-modal distribution of the fibril cross-sectional dimension. The smallest size, 22 Å observed in all samples concurred with the WAXD results of the control and opposite side samples. This smallest and the most abundant occurring size was interpreted as the cellulose elementary microfibril diameter. The intermediate size of 45 Å, which is most pronounced in the tension side sample and consistent with WAXD results for tension side sample, indicates association of neighboring elementary microfibrils to form larger crystallite bundles. The largest size 61 Å observed by SANS was however not observed by WAXD and therefore associated to mesopores. Conclusions: Structure and morphology of tension wood is different from control wood. Cellulose crystallinity increases, lignin content is lower and the appearance of mesopores with 61 Å diameter is observed. Despite the presence of higher crystalline cellulose content in tension side, the lower lignin content and may be combined with the abundance of mesopores, substantially improves enzyme accessibility leading to higher yields in cellulose digestion.

Details

Original languageEnglish
Article number44
JournalBiotechnology for Biofuels
Volume11
Issue number1
Publication statusPublished - 16 Feb 2018
MoE publication typeA1 Journal article-refereed

    Research areas

  • Coalescence of cellulose microfibrils, Poplar, Small-angle neutron scattering, SANS, Tension wood, Wide-angle X-ray diffraction, WAXD

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