Temperature-dependence of rod photoresponses from the aspartate-treated retina of the frog (Rana temporaria)

K. Donner, S. Hemilä, Ari Koskelainen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The effects of temperature changes on rod photoresponses were studied by recording the aspartate-isolated mass receptor potential in the dark-adapted retina of the frog Rana temporaria. The amplitude of saturating responses, indicating the magnitude of the dark current, increased linearly with temperature in the measured range 6–26 °C, extrapolating to zero dark current at O °C. Sensitivity was maximal around 18 °C but the decrease towards lower temperatures was shallow. The results show that rod phototransduction in the frog Rana temporaria is adapted to lower temperatures than in the tropical road Bufo marinus. Responses to dim flashes were, approximately up to peak, well fitted by the same ‘independent activation’ model with four delay stages as have been found to best describe current responses from single toad rods. The kinetics (reciprocal time-to-peak) showed Arrhenius-type temperature-dependence with apparent activation energy 12.4 kcal mol-1 and Q10 = 2.1.
Original languageEnglish
Pages (from-to)535-541
JournalACTA PHYSIOLOGICA
Volume134
Issue number4
DOIs
Publication statusPublished - 1988
MoE publication typeA1 Journal article-refereed

Keywords

  • adaptation
  • amphibians
  • photoreception
  • temperature effects
  • vision

Fingerprint

Dive into the research topics of 'Temperature-dependence of rod photoresponses from the aspartate-treated retina of the frog (Rana temporaria)'. Together they form a unique fingerprint.

Cite this