Tailoring N-Glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae
Research output: Contribution to journal › Article › Scientific › peer-review
The ability to control and adjust the N-glycosylation pathway of Saccharomyces cerevisiae is a key step toward production of therapeutic glycoproteins such as antibodies or erythropoietin. The focus of this chapter is to describe the road from yeast-type N-glycosylation to human-type complex N-glycosylation. The chapter describes the cell engineering and provides the detailed analytical procedures required to perform glycan analysis using MALDI-TOF mass spectrometry.
|Number of pages||15|
|Journal||Methods in molecular biology (Clifton, N.J.)|
|Publication status||Published - 1 Jan 2019|
|MoE publication type||A1 Journal article-refereed|
- Glycan analysis, Glycoengineering, Glycosylation efficiency, Glycosyltransferases, MALDI-TOF MS, S. cerevisiae