Abstract
The ability to control and adjust the N-glycosylation pathway of Saccharomyces cerevisiae is a key step toward production of therapeutic glycoproteins such as antibodies or erythropoietin. The focus of this chapter is to describe the road from yeast-type N-glycosylation to human-type complex N-glycosylation. The chapter describes the cell engineering and provides the detailed analytical procedures required to perform glycan analysis using MALDI-TOF mass spectrometry.
Original language | English |
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Pages (from-to) | 227-241 |
Number of pages | 15 |
Journal | Methods in molecular biology (Clifton, N.J.) |
Volume | 1923 |
DOIs | |
Publication status | Published - 1 Jan 2019 |
MoE publication type | A1 Journal article-refereed |
Keywords
- Glycan analysis
- Glycoengineering
- Glycosylation efficiency
- Glycosyltransferases
- MALDI-TOF MS
- S. cerevisiae
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