Tailoring N-Glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae

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Abstract

The ability to control and adjust the N-glycosylation pathway of Saccharomyces cerevisiae is a key step toward production of therapeutic glycoproteins such as antibodies or erythropoietin. The focus of this chapter is to describe the road from yeast-type N-glycosylation to human-type complex N-glycosylation. The chapter describes the cell engineering and provides the detailed analytical procedures required to perform glycan analysis using MALDI-TOF mass spectrometry.

Original languageEnglish
Pages (from-to)227-241
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume1923
DOIs
Publication statusPublished - 1 Jan 2019
MoE publication typeA1 Journal article-refereed

Keywords

  • Glycan analysis
  • Glycoengineering
  • Glycosylation efficiency
  • Glycosyltransferases
  • MALDI-TOF MS
  • S. cerevisiae

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