Synthesis of enantiopure glycidol derivatives via a one-pot two-step enzymatic cascade

Yu Chang Liu, Yan Liu, Zhong Liu Wu*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)


Styrene monooxygenase (SMO) can catalyze the kinetic resolution of secondary allylic alcohols to provide enantiopure glycidol derivatives. To overcome the low theoretical yield of kinetic resolution, we designed a one-pot two-step enzymatic cascade using prochiral α,β-unsaturated ketones as the substrates. An S-specific ketoreductase ChKRED03 was screened for the efficient bioreduction of the substrates to provide (S)-allylic alcohols, which underwent SMO-catalyzed epoxidation to achieve glycidol derivatives with contiguous stereogenic centers. Excellent enantioselectivity (ee > 99%) and diastereoselectivity (de > 99%) were achieved for the majority of the substrates, and product yields reached up to >99%. This journal is

Original languageEnglish
Pages (from-to)2146-2152
Number of pages7
Issue number7
Publication statusPublished - 21 Feb 2015
MoE publication typeA1 Journal article-refereed


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