Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection

Mohammad Awashra; Pinja Elomaa; Tuomas Ojalehto; Päivi Saavalainen; Ville Jokinen

doi:10.1002/admi.202300596

Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection

Mohammad Awashra^*, Pinja Elomaa, Tuomas Ojalehto, Päivi Saavalainen, Ville Jokinen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

11 Citations (Scopus)

56 Downloads (Pure)

Abstract

Superhydrophilic/superhydrophobic patterned surfaces can be used to create droplet microarrays. A specific challenge with the liquids needed for various biomedical applications, as compared to pure water, is their lower surface tension and potential for contaminating the surfaces through adsorption. Here, a method is shown to create biofluid droplet microarrays using discontinuous dewetting of pure water, an oil protective layer, and finally biofluid exchange with the water droplet array. With this method, a droplet array of a viscous nucleic acid amplification solution can be formed with a low surface tension of 34 mN m⁻¹ and a contact angle of only 76° with the used hydrophobic coating. This droplet array is applied for nucleic acid detection of SARS-CoV-2 virus using strand invasion-based amplification (SIBA) technology. It is shown that by using an array of 10 000 droplets of 50 µm diameter the limit of detection is 1 RNA copy µL⁻¹. The results demonstrate that SIBA on droplet microarrays may be a quantitative technology.

Original language	English
Article number	2300596
Number of pages	9
Journal	Advanced Materials Interfaces
Volume	11
Issue number	1
Early online date	23 Oct 2023
DOIs	https://doi.org/10.1002/admi.202300596
Publication status	Published - 4 Jan 2024
MoE publication type	A1 Journal article-refereed

Funding

This work utilized the cleanroom facilities of Micronova, which is part of the OtaNano national research infrastructure. Imaging was done at the Biomedicum Imaging Unit, Helsinki University, Finland, with the support of Biocenter Finland. This work was funded by the Academy of Finland (#341459). P.E. would also like to acknowledge the Doctoral Programme in Clinical Research for her studentship.

Keywords

biofluids
black silicon
digital nucleic acid amplification
microfluidics
wettability patterning

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/admi.202300596Licence: CC BY

CHEM_Awashra_et_al_Superhydrophilic_Superhydrophobic_2024_Advanced_Materials_InterfacesFinal published version, 2.51 MBLicence: CC BY

Cite this

@article{379319d4f06c4014b9ed680332a0d0f2,

title = "Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection",

abstract = "Superhydrophilic/superhydrophobic patterned surfaces can be used to create droplet microarrays. A specific challenge with the liquids needed for various biomedical applications, as compared to pure water, is their lower surface tension and potential for contaminating the surfaces through adsorption. Here, a method is shown to create biofluid droplet microarrays using discontinuous dewetting of pure water, an oil protective layer, and finally biofluid exchange with the water droplet array. With this method, a droplet array of a viscous nucleic acid amplification solution can be formed with a low surface tension of 34 mN m−1 and a contact angle of only 76° with the used hydrophobic coating. This droplet array is applied for nucleic acid detection of SARS-CoV-2 virus using strand invasion-based amplification (SIBA) technology. It is shown that by using an array of 10 000 droplets of 50 µm diameter the limit of detection is 1 RNA copy µL−1. The results demonstrate that SIBA on droplet microarrays may be a quantitative technology.",

keywords = "biofluids, black silicon, digital nucleic acid amplification, microfluidics, wettability patterning",

author = "Mohammad Awashra and Pinja Elomaa and Tuomas Ojalehto and P{\"a}ivi Saavalainen and Ville Jokinen",

note = "Funding Information: This work utilized the cleanroom facilities of Micronova, which is part of the OtaNano national research infrastructure. Imaging was done at the Biomedicum Imaging Unit, Helsinki University, Finland, with the support of Biocenter Finland. This work was funded by the Academy of Finland (\#341459). P.E. would also like to acknowledge the Doctoral Programme in Clinical Research for her studentship. Publisher Copyright: {\textcopyright} 2023 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.",

year = "2024",

month = jan,

day = "4",

doi = "10.1002/admi.202300596",

language = "English",

volume = "11",

journal = "Advanced Materials Interfaces",

issn = "2196-7350",

publisher = "Wiley",

number = "1",

}

TY - JOUR

T1 - Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection

AU - Awashra, Mohammad

AU - Elomaa, Pinja

AU - Ojalehto, Tuomas

AU - Saavalainen, Päivi

AU - Jokinen, Ville

N1 - Funding Information: This work utilized the cleanroom facilities of Micronova, which is part of the OtaNano national research infrastructure. Imaging was done at the Biomedicum Imaging Unit, Helsinki University, Finland, with the support of Biocenter Finland. This work was funded by the Academy of Finland (#341459). P.E. would also like to acknowledge the Doctoral Programme in Clinical Research for her studentship. Publisher Copyright: © 2023 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.

PY - 2024/1/4

Y1 - 2024/1/4

N2 - Superhydrophilic/superhydrophobic patterned surfaces can be used to create droplet microarrays. A specific challenge with the liquids needed for various biomedical applications, as compared to pure water, is their lower surface tension and potential for contaminating the surfaces through adsorption. Here, a method is shown to create biofluid droplet microarrays using discontinuous dewetting of pure water, an oil protective layer, and finally biofluid exchange with the water droplet array. With this method, a droplet array of a viscous nucleic acid amplification solution can be formed with a low surface tension of 34 mN m−1 and a contact angle of only 76° with the used hydrophobic coating. This droplet array is applied for nucleic acid detection of SARS-CoV-2 virus using strand invasion-based amplification (SIBA) technology. It is shown that by using an array of 10 000 droplets of 50 µm diameter the limit of detection is 1 RNA copy µL−1. The results demonstrate that SIBA on droplet microarrays may be a quantitative technology.

AB - Superhydrophilic/superhydrophobic patterned surfaces can be used to create droplet microarrays. A specific challenge with the liquids needed for various biomedical applications, as compared to pure water, is their lower surface tension and potential for contaminating the surfaces through adsorption. Here, a method is shown to create biofluid droplet microarrays using discontinuous dewetting of pure water, an oil protective layer, and finally biofluid exchange with the water droplet array. With this method, a droplet array of a viscous nucleic acid amplification solution can be formed with a low surface tension of 34 mN m−1 and a contact angle of only 76° with the used hydrophobic coating. This droplet array is applied for nucleic acid detection of SARS-CoV-2 virus using strand invasion-based amplification (SIBA) technology. It is shown that by using an array of 10 000 droplets of 50 µm diameter the limit of detection is 1 RNA copy µL−1. The results demonstrate that SIBA on droplet microarrays may be a quantitative technology.

KW - biofluids

KW - black silicon

KW - digital nucleic acid amplification

KW - microfluidics

KW - wettability patterning

UR - https://www.scopus.com/pages/publications/85174594035

U2 - 10.1002/admi.202300596

DO - 10.1002/admi.202300596

M3 - Article

AN - SCOPUS:85174594035

SN - 2196-7350

VL - 11

JO - Advanced Materials Interfaces

JF - Advanced Materials Interfaces

IS - 1

M1 - 2300596

ER -

Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection

Abstract

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

CELLREP: Cell-Repellent Superhydrophobic Surfaces

OtaNano

OtaNano - Nanofab

New CDC and FDA Study Findings Reported from Aalto University (Superhydrophilic/superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection)

Cite this

Superhydrophilic/Superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection

Abstract

Funding

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

CELLREP: Cell-Repellent Superhydrophobic Surfaces

Equipment

OtaNano

OtaNano - Nanofab

Press/Media

New CDC and FDA Study Findings Reported from Aalto University (Superhydrophilic/superhydrophobic Droplet Microarrays of Low Surface Tension Biofluids for Nucleic Acid Detection)

Cite this