Projects per year
Abstract
DNA origami nanostructures have attracted significant attention as an innovative tool in a variety of research areas, spanning from nanophotonics to bottom-up nanofabrication. However, the use of DNA origami is often restricted by their rather limited structural stability in application-specific conditions. The structural integrity of DNA origami is known to be superstructure-dependent, and the integrity is influenced by various external factors, for example cation concentration, temperature, and presence of nucleases. Given the necessity to functionalize DNA origami also with non-water-soluble entities, it is important to acquire knowledge of the structural stability of DNA origami in various organic solvents. Therefore, we herein systematically investigate the post-folding DNA origami stability in a variety of polar, water-miscible solvents, including acetone, ethanol, DMF, and DMSO. Our results suggest that the structural integrity of DNA origami in organic solvents is both superstructure-dependent and dependent on the properties of the organic solvent. In addition, DNA origami are generally more resistant to added organic solvents in folding buffer compared to that in deionized water. DNA origami stability can be maintained in up to 25–40% DMF or DMSO and up to 70–90% acetone or ethanol, with the highest overall stability observed in acetone. By rationally selecting both the DNA origami design and the solvent, the DNA origami stability can be maintained in high concentrations of organic solvents, which paves the way for more extensive use of non-water-soluble compounds for DNA origami functionalization and complexation.
Original language | English |
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Pages (from-to) | 13407-13415 |
Number of pages | 9 |
Journal | Nanoscale |
Volume | 16 |
Issue number | 28 |
Early online date | 19 Jun 2024 |
DOIs | |
Publication status | Published - 28 Jul 2024 |
MoE publication type | A1 Journal article-refereed |
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Dive into the research topics of 'Structural stability of DNA origami nanostructures in organic solvents'. Together they form a unique fingerprint.Projects
- 2 Active
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LIBER Kostiainen: Life-like hybrid materials Kostiainen
Kostiainen, M. (Principal investigator), Luotonen, O. (Project Member), George, L. (Project Member), Wierzchowiecka, J. (Project Member) & Zhou, Y. (Project Member)
01/01/2022 → 31/12/2024
Project: Academy of Finland: Other research funding
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ProCrystal (ERC): Multicomponent Protein Cage Co-Crystals
Kostiainen, M. (Principal investigator), Ahmed, A. (Project Member), De, S. (Project Member), Liu, Q. (Project Member), Zhou, Y. (Project Member), Enlund, E. (Project Member) & Saarinen, S. (Project Member)
01/09/2021 → 31/08/2026
Project: EU: ERC grants
Equipment
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Bioeconomy Research Infrastructure
Seppälä, J. (Manager)
School of Chemical EngineeringFacility/equipment: Facility
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