The main target of a biorefinery pretreatment process is to break down the lignin-reinforced plant cell wall structure prior to enzymatic hydrolysis of polysaccharides to fermentable sugars. Various physico-chemical alterations occur in lignin during the biomass pretreatment, but effects of those structural changes on subsequent enzymatic hydrolysis have remained ambiguous. We review the reinforcing and detrimental lignin-enzyme interactions and their underlying mechanisms, and use this structure-function information to assess critical features of current and emerging pretreatment technologies. Our perspective is that truly multidisciplinary research is needed to develop pretreatments that render lignin non-inhibiting to enzymes and with high potential for further valorisation.
|Journal||Nordic Pulp & Paper Research Journal|
|Publication status||Published - 2017|
|MoE publication type||A1 Journal article-refereed|
Sipponen, M., Rahikainen, J., Leskinen, T., Pihlajaniemi, V., Mattinen, M-L., Lange, H., Crestini, C., & Österberg, M. (2017). Structural changes of lignin in biorefinery pretreatments and consequences to enzyme-lignin interactions. Nordic Pulp & Paper Research Journal, 32(4), 547-568. https://doi.org/10.3183/npprj-2017-32-04_p550-571_sipponen