Stabilization of cutinase by covalent attachment on magnetic nanoparticles and improvement of its catalytic activity by ultrasonication
Research output: Contribution to journal › Article › Scientific › peer-review
- Institute of Chemical Technology
This paper reports on stabilization of serine cutinase activity by immobilizing it through cross linking with glutaraldehyde on magnetic nanoparticles (Fe-NPs) and intensification of catalytic activity by ultrasonic treatment. The optimum parameters were cross linking with 10.52 mM glutaraldehyde for 90 min using 1:2 (w/w) ratio of enzyme:Fe-NPs. The characterization of cutinase-Fe-NPs was done by different instrumental analysis. Ultrasonic power showed a beneficial effect on the activity of free and immobilized cutinase at 5.76 and 7.63 W, respectively, after 12 min. Immobilization and ultrasonic treatment led to increments in kinetic parameters (K m and V max ) along with noticeable changes in the secondary structural fractions of cutinase. Cutinase-Fe-NPs showed augmented pH (4–8) and thermal stability (40–60 °C). Considerably higher thermal inactivation kinetic constants (k d , t 1/2 and D-value) and thermodynamic constants (E d , ΔH°, ΔG° and ΔS°) highlighted superior thermostability of cutinase-Fe-NPs. Cutinase-Fe-NPs and ultrasound treated cutinase-Fe-NPs retained 61.88% and 38.76% activity during 21-day storage, and 82.82 and 80.69% activity after fifth reusability cycle, respectively.
|Number of pages||12|
|Publication status||Published - 1 Jul 2019|
|MoE publication type||A1 Journal article-refereed|
- Cutinase, Kinetics, Magnetic nanoparticles, Thermal stability, Thermodynamics, Ultrasonic treatment