Stability of DNA Origami Nanostructures in Physiological Media: The Role of Molecular Interactions

Veikko Linko*, Adrian Keller*

*Corresponding author for this work

Research output: Contribution to journalReview Articlepeer-review

14 Citations (Scopus)
22 Downloads (Pure)


Programmable, custom-shaped, and nanometer-precise DNA origami nanostructures have rapidly emerged as prospective and versatile tools in bionanotechnology and biomedicine. Despite tremendous progress in their utilization in these fields, essential questions related to their structural stability under physiological conditions remain unanswered. Here, DNA origami stability is explored by strictly focusing on distinct molecular-level interactions. In this regard, the fundamental stabilizing and destabilizing ionic interactions as well as interactions involving various enzymes and other proteins are discussed, and their role in maintaining, modulating, or decreasing the structural integrity and colloidal stability of DNA origami nanostructures is summarized. Additionally, specific issues demanding further investigation are identified. This review – through its specific viewpoint – may serve as a primer for designing new, stable DNA objects and for adapting their use in applications dealing with physiological media.

Original languageEnglish
Article number2301935
Number of pages15
Issue number34
Early online date24 Apr 2023
Publication statusPublished - 23 Aug 2023
MoE publication typeA2 Review article, Literature review, Systematic review


  • DNA nanotechnology
  • enzymes
  • ions
  • nucleases
  • stability


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